Application
Fig1: Immunocytochemical staining of Hela cells using anti-MLCK rabbit polyclonal antibody.Application
Fig2: Immunocytochemical staining of NIH/3T3 cells using anti-MLCK rabbit polyclonal antibody.Application
Fig3： Immunocytochemical staining of SHG-44 cells using anti-MLCK rabbit polyclonal antibody.Application
Fig4： Immunohistochemical analysis of paraffin- embedded mouse smooth muscle tissue using anti-MLCK rabbit polyclonal antibody.Application
Fig5： Flow cytometric analysis of N2A cells with MLCK antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated Goat anti rabbit IgG was used as the secondary antibody.| Product Name | MLCK |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | deglutamylated form antibody DKFZp686I10125 antibody EC 2.7.11.18 antibody FLJ12216 antibody Kinase related protein antibody Kinase-related protein antibody KRP antibody MLCK antibody MLCK1 antibody MLCK108 antibody MLCK210 antibody MSTP083 antibody MYLK antibody MYLK_HUMAN antibody MYLK1 antibody Myosin light chain kinase antibody Myosin light polypeptide kinase antibody OTTHUMP00000180642 antibody OTTHUMP00000180643 antibody smMLCK antibody smooth muscle antibody Smooth muscle myosin light chain kinase antibody Telokin antibody |
| Product description | The Ca2+/calmodulin-dependent protein kinases (CaM kinases) are a structurally related subfamily of serine/threonine kinases that includes CaMKI, CaMKII, CaMKIV and myosin light chain kinases (MYLK, also designated MLCK). The MYLK kinases phosphorylate myosin regulatory light chains to catalyze myosin interaction with actin filaments resulting in contractile activity. Non-muscle, smooth muscle and skeletal/cardiac muscle MYLK isoforms exist. The MYLK gene (also designated MYLK1) encodes both smooth muscle and non-muscle isoforms as well as telokin, a small C-terminal isoform expressed only in smooth muscle with the capacity to stabilize unphosphorylated myosin filaments. Multiple transcript variants are described for the MYLK gene. Smooth-muscle and non-muscle MYLK isoforms are expressed in a wide variety of adult and fetal tissues. The skeletal/cardiac muscle isoforms of MYLK are encoded by a separate gene, MYLK2 (also designated skMLCK). MYLK appears to be a target for PAKs (p21-activated kinases). PAK1 interaction with MYLK results in a decrease in MYLK activity and myosin light chain phosphorylation. |
| Immunogen | peptide |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICCIHCFC |
| ICC:1:50-1:200 ICC:1:50-1:200 FC:1:50-1:100 |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Alternative Names | deglutamylated form antibody DKFZp686I10125 antibody EC 2.7.11.18 antibody FLJ12216 antibody Kinase related protein antibody Kinase-related protein antibody KRP antibody MLCK antibody MLCK1 antibody MLCK108 antibody MLCK210 antibody MSTP083 antibody MYLK antibody MYLK_HUMAN antibody MYLK1 antibody Myosin light chain kinase antibody Myosin light polypeptide kinase antibody OTTHUMP00000180642 antibody OTTHUMP00000180643 antibody smMLCK antibody smooth muscle antibody Smooth muscle myosin light chain kinase antibody Telokin antibody |
|---|---|
| Cellular Localization | Cytoplasm, Cleavage furrow, Cell projection |
| SwissProt ID | Q15746 |
|---|
Application
Fig1: Immunocytochemical staining of Hela cells using anti-MLCK rabbit polyclonal antibody.
Application
Fig2: Immunocytochemical staining of NIH/3T3 cells using anti-MLCK rabbit polyclonal antibody.
Application
Fig3： Immunocytochemical staining of SHG-44 cells using anti-MLCK rabbit polyclonal antibody.
Application
Fig4： Immunohistochemical analysis of paraffin- embedded mouse smooth muscle tissue using anti-MLCK rabbit polyclonal antibody.
Application
Fig5： Flow cytometric analysis of N2A cells with MLCK antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated Goat anti rabbit IgG was used as the secondary antibody.| Positive Control | Hela, NIH/3T3, SHG-44, mouse smooth muscle tissue. |
|---|---|
| Application Notes | ICC:1:50-1:200 ICC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
