Application
Fig1： Immunocytochemical staining of Hela cells using anti-SMAD3 rabbit polyclonal antibody.Application
Fig2： Immunocytochemical staining of HCT116 cells using anti-SMAD3 rabbit polyclonal antibody.Application
Fig3： Immunocytochemical staining of HepG2 cells using anti-SMAD3 rabbit polyclonal antibody.Application
Fig4： Immunohistochemical analysis of paraffin- embedded human lung carcinoma tissue using anti-SMAD3 rabbit polyclonal antibody.Application
Fig5： Immunohistochemical analysis of paraffin- embedded human liver tissue using anti-SMAD3 rabbit polyclonal antibody.Application
Fig6： Immunohistochemical analysis of paraffin- embedded human colon cancer tissue using anti-SMAD3 rabbit polyclonal antibody.Application
Fig7： Immunohistochemical analysis of paraffin- embedded human breast carcinoma tissue using anti-SMAD3 rabbit polyclonal antibody.Application
Fig8： Immunohistochemical analysis of paraffin- embedded human stomach carcinoma tissue using anti-SMAD3 rabbit polyclonal antibody.Application
Fig9： Flow cytometric analysis of SK-BR-3 cells with SMAD3 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated Goat anti rabbit IgG was used as the secondary antibody.| Product Name | SMAD3 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | DKFZP586N0721 antibody DKFZp686J10186 antibody hMAD 3 antibody hMAD-3 antibody hSMAD3 antibody HSPC193 antibody HST17436 antibody JV15 2 antibody JV15-2 antibody JV152 antibody LDS1C antibody LDS3 antibody MAD (mothers against decapentaplegic Drosophila) homolog 3 antibody MAD homolog 3 antibody Mad homolog JV15 2 antibody Mad protein homolog antibody MAD, mothers against decapentaplegic homolog 3 antibody Mad3 antibody MADH 3 antibody MADH3 antibody MGC60396 antibody Mothers against decapentaplegic homolog 3 antibody Mothers against DPP homolog 3 antibody SMA and MAD related protein 3 antibody SMAD 3 antibody SMAD antibody SMAD family member 3 antibody SMAD, mothers against DPP homolog 3 antibody Smad3 antibody SMAD3_HUMAN antibody |
| Product description | Smad proteins, the mammalian homologs of the Drosophila Mothers against dpp (Mad) have been implicated as downstream effectors of TGFβ/BMP signaling. Smad1 (also designated Madr1 or JV4-1), Smad5 and mammalian Smad8 (also designated Smad9 or MADH6) are effectors of BMP2 and BMP4 function while Smad2 (also designated Madr2 or JV18-1) and Smad3 are involved in TGFβ and activin-mediated growth modulation. Smad4 (also designated DPC4) has been shown to mediate all of the above activities through interaction with various Smad family members. Smad6 and Smad7 regulate the response to activin/TGFβ signaling by interfering with TGFβ-mediated phosphorylation of other Smad family members. |
| Immunogen | peptide |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBICCIHCFC |
| ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 WB:1:500-1:1,000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | DKFZP586N0721 antibody
DKFZp686J10186 antibody
hMAD 3 antibody
hMAD-3 antibody
hSMAD3 antibody
HSPC193 antibody
HST17436 antibody
JV15 2 antibody
JV15-2 antibody
JV152 antibody
LDS1C antibody
LDS3 antibody
MAD (mothers against decapentaplegic Drosophila) homolog 3 antibody
MAD homolog 3 antibody
Mad homolog JV15 2 antibody
Mad protein homolog antibody
MAD mothers against decapentaplegic homolog 3 antibody Mad3 antibody MADH 3 antibody MADH3 antibody MGC60396 antibody Mothers against decapentaplegic homolog 3 antibody Mothers against DPP homolog 3 antibody SMA and MAD related protein 3 antibody SMAD 3 antibody SMAD antibody SMAD family member 3 antibody SMAD mothers against DPP homolog 3 antibody Smad3 antibody SMAD3_HUMAN antibody |
|---|---|
| Molecular Weight(MW) | 48kDa |
| Cellular Localization | Cytoplasm, Nucleus |
| SwissProt ID | P84022 |
|---|
Application
Fig1： Immunocytochemical staining of Hela cells using anti-SMAD3 rabbit polyclonal antibody.
Application
Fig2： Immunocytochemical staining of HCT116 cells using anti-SMAD3 rabbit polyclonal antibody.
Application
Fig3： Immunocytochemical staining of HepG2 cells using anti-SMAD3 rabbit polyclonal antibody.
Application
Fig4： Immunohistochemical analysis of paraffin- embedded human lung carcinoma tissue using anti-SMAD3 rabbit polyclonal antibody.
Application
Fig5： Immunohistochemical analysis of paraffin- embedded human liver tissue using anti-SMAD3 rabbit polyclonal antibody.
Application
Fig6： Immunohistochemical analysis of paraffin- embedded human colon cancer tissue using anti-SMAD3 rabbit polyclonal antibody.
Application
Fig7： Immunohistochemical analysis of paraffin- embedded human breast carcinoma tissue using anti-SMAD3 rabbit polyclonal antibody.
Application
Fig8： Immunohistochemical analysis of paraffin- embedded human stomach carcinoma tissue using anti-SMAD3 rabbit polyclonal antibody.
Application
Fig9： Flow cytometric analysis of SK-BR-3 cells with SMAD3 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated Goat anti rabbit IgG was used as the secondary antibody.| Positive Control | Hela, HCT116, HepG2, human colon cancer tissue, human lung carcinoma tissue, human breast carcinoma tissue, human liver tissue, human stomach carcinoma tissue. |
|---|---|
| Application Notes | ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 WB:1:500-1:1,000 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
