Application
Fig1: Western blot analysis on NCCIT(1) and Hela(2) cell lysates using anti-Pygopus 2 rabbit polyclonal antibody.Application
Fig2： Flow cytometric analysis of MCF-7 cells with Pygopus 2 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated Goat anti rabbit IgG was used as the secondary antibody.| Product Name | Pygopus 2 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | 1190004M21Rik antibody FLJ33226 antibody PP7910 antibody PYGO2 antibody Pygo2 protein antibody PYGO2_HUMAN antibody Pygopus 2 antibody pygopus homolog 2 (Drosophila) antibody Pygopus homolog 2 antibody |
| Product description | Pygopus 2, also known as PYGO2, is a 406 amino acid protein that is the human homolog of the Drosophila pygopus protein. Localized to the nucleus, Pygopus 2 contains one PHD finger that interacts with the homology domain of the Wnt signaling protein Bcl-9. This interaction joins Pygopus 2 with the ∫-catenin/TCF complex (a crucial complex in Wnt signaling), thereby allowing ∫-catenin to transcriptionally activate Wnt target genes. Association of Pygopus 2 with proteins involved in the Wnt signaling pathway is thought to regulate proper signal transduction, as absence of the Pygopus 2/∫-catenin interaction may play a role in development of B-cell malignancies. In addition, Pygopus 2 expression is upregulated in and required for the growth of breast cancer cells, suggesting a crucial role in carcinogenesis. |
| Immunogen | peptide |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBFC |
| WB:1:1,000 FC:1:50-1:100 |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Alternative Names | 1190004M21Rik antibody FLJ33226 antibody PP7910 antibody PYGO2 antibody Pygo2 protein antibody PYGO2_HUMAN antibody Pygopus 2 antibody pygopus homolog 2 (Drosophila) antibody Pygopus homolog 2 antibody |
|---|---|
| Molecular Weight(MW) | 41kDa |
| Cellular Localization | Nucleus |
| SwissProt ID | Q9BRQ0 |
|---|
Application
Fig1: Western blot analysis on NCCIT(1) and Hela(2) cell lysates using anti-Pygopus 2 rabbit polyclonal antibody.
Application
Fig2： Flow cytometric analysis of MCF-7 cells with Pygopus 2 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated Goat anti rabbit IgG was used as the secondary antibody.| Positive Control | Hela, NCCIT. |
|---|---|
| Application Notes | WB:1:1,000 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
