Application
Fig1: Western blot analysis of Occludin on mouse kidney (1) and human kidney (2) tissue lysates using anti-Occludin antibody at 1/1,000 dilution.Application
Fig2: ICC staining Occludin in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining Occludin in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: Immunohistochemical analysis of paraffin-embedded human lung tissue using anti-Occludin antibody. Counter stained with hematoxylin.Application
Fig5: Immunohistochemical analysis of paraffin-embedded mouse lung tissue using anti-Occludin antibody. Counter stained with hematoxylin.Application
Fig6: Flow cytometric analysis of HepG2 cells with Occludin antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.| Product Name | Occludin |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | BLCPMG antibody FLJ08163 antibody FLJ18079 antibody FLJ77961 antibody FLJ94056 antibody MGC34277 antibody Occludin antibody Ocln antibody OCLN_HUMAN antibody Phosphatase 1 regulatory subunit 115 antibody PPP1R115 antibody PTORCH1 antibody Tight junction protein occludin antibody |
| Product description | This gene encodes an integral membrane protein that is required for cytokine-induced regulation of the tight junction paracellular permeability barrier. Mutations in this gene are thought to be a cause of band-like calcification with simplified gyration and polymicrogyria (BLC-PMG), an autosomal recessive neurologic disorder that is also known as pseudo-TORCH syndrome. Alternative splicing results in multiple transcript variants. A related pseudogene is present 1.5 Mb downstream on the q arm of chromosome 5. |
| Immunogen | Synthetic peptide within human Occludin aa 1-50 conjugated to Keyhole Limpet Haemocyanin (KLH). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBICCIHCFC |
| WB:1:500-2,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | BLCPMG antibody FLJ08163 antibody FLJ18079 antibody FLJ77961 antibody FLJ94056 antibody MGC34277 antibody Occludin antibody Ocln antibody OCLN_HUMAN antibody Phosphatase 1 regulatory subunit 115 antibody PPP1R115 antibody PTORCH1 antibody Tight junction protein occludin antibody |
|---|---|
| Molecular Weight(MW) | 59 kDa |
| Cellular Localization | Cell membrane. |
Application
Fig1: Western blot analysis of Occludin on mouse kidney (1) and human kidney (2) tissue lysates using anti-Occludin antibody at 1/1,000 dilution.
Application
Fig2: ICC staining Occludin in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining Occludin in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded human lung tissue using anti-Occludin antibody. Counter stained with hematoxylin.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded mouse lung tissue using anti-Occludin antibody. Counter stained with hematoxylin.
Application
Fig6: Flow cytometric analysis of HepG2 cells with Occludin antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.| Positive Control | mouse kidney and human kidney tissue lysates, HepG2, Hela, A549, human lung tissue, mouse lung tissue. |
|---|---|
| Application Notes | WB:1:500-2,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 0.5%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide. |
