Application
Fig1: Western blot analysis on mouse testis lysates using anti-YB1 rabbit polyclonal antibody.Application
Fig2： Immunocytochemical staining of Hela cells using anti-YB1 rabbit polyclonal antibody.Application
Fig3： Immunohistochemical analysis of paraffin- embedded mouse testis tissue using anti-YB1 rabbit polyclonal antibody.Application
Fig4： Immunohistochemical analysis of paraffin- embedded mouse ovary tissue using anti-YB1 rabbit polyclonal antibody.Application
Fig5： Flow cytometric analysis of Hela cells with YB1 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated Goat anti rabbit IgG was used as the secondary antibody.| Product Name | YBOX1 / YB-1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | BP 8 antibody CBF-A antibody CCAAT binding transcription factor I subunit A antibody CCAAT-binding transcription factor I subunit A antibody CSDA2 antibody CSDB antibody DBPB antibody DNA binding protein B antibody DNA-binding protein B antibody EFI-A antibody Enhancer factor I subunit A antibody MDR NF1 antibody MGC104858 antibody MGC110976 antibody MGC117250 antibody NSEP 1 antibody NSEP1 antibody Nuclease sensitive element binding protein 1 antibody Nuclease-sensitive element-binding protein 1 antibody p50 antibody Q15905 antibody Y-box binding protein 1 antibody Y-box transcription factor antibody Y-box-binding protein 1 antibody YB 1 antibody YB-1 antibody YBOX1_HUMAN antibody YBX 1 antibody ybx1 antibody |
| Product description | MSY2 and YB-2 (MSY3,4) belong to the Y-box family of multifunctional proteins that regulate both transcription and translation. Y-box proteins interact with a wide variety of nucleic acid structures to act as transcription factors and mRNA masking proteins. The modular structure of Y-box proteins includes a highly conserved N-terminal cold-shock domain (CSD, equivalent to the bacterial cold-shock proteins) and four basic C-terminal domains containing arginine clusters and aromatic residues. MSY2 is expressed in testis and ovary where it may repress translation of parental mRNA. The gene encoding human MSY2 maps to chromosome 17p11.2-13.1. YB-2 (MSY3,4 in mouse) is also known as DNA binding protein A and is highly expressed in the testis, heart and muscle. MSY2 and YB-2 bind to the consensus sequence 5'-UCCAUCA-3' contained in the Y-box element. |
| Immunogen | peptide |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBICCIHCFC |
| WB:1:1,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | BP 8 antibody CBF-A antibody CCAAT binding transcription factor I subunit A antibody CCAAT-binding transcription factor I subunit A antibody CSDA2 antibody CSDB antibody DBPB antibody DNA binding protein B antibody DNA-binding protein B antibody EFI-A antibody Enhancer factor I subunit A antibody MDR NF1 antibody MGC104858 antibody MGC110976 antibody MGC117250 antibody NSEP 1 antibody NSEP1 antibody Nuclease sensitive element binding protein 1 antibody Nuclease-sensitive element-binding protein 1 antibody p50 antibody Q15905 antibody Y-box binding protein 1 antibody Y-box transcription factor antibody Y-box-binding protein 1 antibody YB 1 antibody YB-1 antibody YBOX1_HUMAN antibody YBX 1 antibody ybx1 antibody |
|---|---|
| Molecular Weight(MW) | 40kDa |
| Cellular Localization | Cytoplasm, Nucleus |
| SwissProt ID | Q9Y2T7 |
|---|
Application
Fig1: Western blot analysis on mouse testis lysates using anti-YB1 rabbit polyclonal antibody.
Application
Fig2： Immunocytochemical staining of Hela cells using anti-YB1 rabbit polyclonal antibody.
Application
Fig3： Immunohistochemical analysis of paraffin- embedded mouse testis tissue using anti-YB1 rabbit polyclonal antibody.
Application
Fig4： Immunohistochemical analysis of paraffin- embedded mouse ovary tissue using anti-YB1 rabbit polyclonal antibody.
Application
Fig5： Flow cytometric analysis of Hela cells with YB1 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated Goat anti rabbit IgG was used as the secondary antibody.| Positive Control | Hela, mouse testis tissue, mouse ovary tissue. |
|---|---|
| Application Notes | WB:1:1,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
