WB
Western blot analysis of Peroxiredoxin 2 on different lysates with Rabbit anti-Peroxiredoxin 2 antibody at 1/1,000 dilution. Lane 1: HeLa cell lysate, Lane 2: PC-12 cell lysate, Lane 3: A549 cell lysate, Lane 4: 293T cell lysate, Lysates/proteins at 20 µg/Lane. Exposure time: 15 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/100,000 dilution was used for 1 hour at room temperature.WB
Western blot analysis of Peroxiredoxin 2 on different lysates with Rabbit anti-Peroxiredoxin 2 antibody at 1/1,000 dilution. Lane 1: PC-12 cell lysate (10 µg/Lane), Lane 2: HeLa cell lysate (10 µg/Lane), Lane 3: Mouse heart tissue lysate (20 µg/Lane), Exposure time: 2 minutes; 15% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/300,000 dilution was used for 1 hour at room temperature.ICC/IF
ICC staining Peroxiredoxin 2/PRDX2 in SHG-44 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.ICC/IF
ICC staining Peroxiredoxin 2/PRDX2 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.IHC
Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Peroxiredoxin 2/PRDX2 antibody. Counter stained with hematoxylin.IHC
Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Peroxiredoxin 2/PRDX2 antibody. Counter stained with hematoxylin.IHC
Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Peroxiredoxin 2/PRDX2 antibody. Counter stained with hematoxylin.IHC
Immunohistochemical analysis of paraffin-embedded mouse prostate tissue using anti-Peroxiredoxin 2/PRDX2 antibody. Counter stained with hematoxylin.IHC
Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-Peroxiredoxin 2/PRDX2 antibody. Counter stained with hematoxylin.| Product Name | Peroxiredoxin 2 Rabbit Polyclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | The peroxiredoxin (PRX) family comprises six antioxidant proteins, PRX I, II, III, IV, V and VI, which protect cells from reactive oxygen species (ROS) by preventing the metal-catalyzed oxidation of enzymes. The PRX proteins primarily utilize thioredoxin as the electron donor for antioxidation, although they are fairly promiscuous with regard to the hydroperoxide substrate. In addition to protection from ROS, peroxiredoxins are also involved in cell proliferation, differentiation and gene expression. PRX I, II, IV and VI show diffuse cytoplasmic localization。The human PRX I gene encodes a protein that is expressed in several tissues, including liver, kidney, testis, lung and nervous system. PRX II is expressed in testis, while PRX III shows expression in lung. PRX I, II and III are overexpressed in breast cancer and may be involved in its development or progression. Upregulated protein levels of PRX I and II in Alzheimer's disease (AD) and Down syndrome (DS) indicate the involvement of PRX I and II in their pathogenesis. |
| Immunogen | Synthetic peptide within Human PRDX2 aa 149-198 / 198. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:1000-1:5000 ICC/IF:1:50-1:200 IHC:1:100-1:500 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | Epididymis secretory sperm binding protein Li 2a antibody HEL S 2a antibody MGC4104 antibody Natural killer cell enhancing factor B antibody Natural killer cell-enhancing factor B antibody Natural Killer Enhancing Factor B antibody NKEF B antibody NKEF-B antibody NKEFB antibody Peroxiredoxin-2 antibody PRDX 2 antibody PRDX2 antibody PRDX2_HUMAN antibody PrP antibody PRX2 antibody PRXII antibody PTX1 antibody TDPX1 antibody Thiol Specific Antioxidant 1 antibody Thiol specific antioxidant protein antibody Thiol-specific antioxidant protein antibody Thioredoxin Dependent Peroxide Reductase 1 antibody Thioredoxin peroxidase 1 antibody Thioredoxin-dependent peroxide reductase 1 antibody Torin antibody TPX1 antibody TSA antibody |
|---|---|
| Molecular Weight(MW) | 22 kDa |
| Cellular Localization | Cytoplasm. |
WB
Western blot analysis of Peroxiredoxin 2 on different lysates with Rabbit anti-Peroxiredoxin 2 antibody at 1/1,000 dilution. Lane 1: HeLa cell lysate, Lane 2: PC-12 cell lysate, Lane 3: A549 cell lysate, Lane 4: 293T cell lysate, Lysates/proteins at 20 µg/Lane. Exposure time: 15 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/100,000 dilution was used for 1 hour at room temperature.
WB
Western blot analysis of Peroxiredoxin 2 on different lysates with Rabbit anti-Peroxiredoxin 2 antibody at 1/1,000 dilution. Lane 1: PC-12 cell lysate (10 µg/Lane), Lane 2: HeLa cell lysate (10 µg/Lane), Lane 3: Mouse heart tissue lysate (20 µg/Lane), Exposure time: 2 minutes; 15% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/300,000 dilution was used for 1 hour at room temperature.
ICC/IF
ICC staining Peroxiredoxin 2/PRDX2 in SHG-44 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC/IF
ICC staining Peroxiredoxin 2/PRDX2 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
IHC
Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Peroxiredoxin 2/PRDX2 antibody. Counter stained with hematoxylin.
IHC
Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Peroxiredoxin 2/PRDX2 antibody. Counter stained with hematoxylin.
IHC
Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Peroxiredoxin 2/PRDX2 antibody. Counter stained with hematoxylin.
IHC
Immunohistochemical analysis of paraffin-embedded mouse prostate tissue using anti-Peroxiredoxin 2/PRDX2 antibody. Counter stained with hematoxylin.
IHC
Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-Peroxiredoxin 2/PRDX2 antibody. Counter stained with hematoxylin.| Application Notes | WB:1:1000-1:5000 ICC/IF:1:50-1:200 IHC:1:100-1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
