WB
Western blot analysis of HSP47 on different lysates with Rabbit anti-HSP47 antibody at 1/1,000 dilution. Lane 1: HeLa cell lysate, Lane 2: NIH/3T3 cell lysate, Lane 3: C2C12 cell lysate, Lysates/proteins at 20 µg/Lane. Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.IHC
Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-HSP47 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/800) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.| Product Name | HSP47 Rabbit Polyclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Heat shock protein 47, also known as SERPINH1 is a serpin which serves as a human chaperone protein for collagen. This protein is a member of the serpin superfamily of serine proteinase inhibitors. Its expression is induced by heat shock. HSP47 is expressed by cells present in Endoplasmic Reticulum. These cells synthesize and secrete type I and type II collagen. The protein localizes to the endoplasmic reticulum lumen and binds collagen; thus it is thought to be a molecular chaperone involved in the maturation of collagen molecules. Autoantibodies to this protein have been found in patients with rheumatoid arthritis. Heat shock protein 47 has been shown to interact with collagens I, II, III, IV and V. In the ER, HSP47 interacts with and stabilizes correctly-folded procollagen. Nucleotide polymorphisms may be associated with preterm birth and Osteogenesis Imperfecta type X. Serpin-H1 is up-regulated in several cancers including squamous cell carcinoma, breast and prostate carcinomas. Expression in tumors drives malignant growth and invasion by enhancing deposition of extracellular matrix proteins. |
| Immunogen | Recombinant protein within Human HSP47 aa 72-318 / 418. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| WB:1:500-1:2000 IHC:1:100-1:500 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | 47 kDa heat shock protein antibody
47 kDa heat shock protein precursor antibody
Arsenic transactivated protein 3 antibody Arsenic-transactivated protein 3 antibody AsTP 3 antibody AsTP3 antibody CBP 1 antibody CBP 2 antibody CBP1 antibody CBP2 antibody Cell proliferation-inducing gene 14 protein antibody Collagen binding protein 1 antibody Collagen binding protein 2 antibody Collagen binding protein antibody Collagen-binding protein 2 antibody Collagen-binding protein antibody Colligen antibody Colligin 1 antibody Colligin 2 antibody Colligin antibody colligin-1 antibody colligin-2 antibody gp46 antibody Heat shock protein 47 antibody Heat-shock protein 47 antibody HGNC 1547 antibody Hsp 47 antibody J6 antibody OI10 antibody PIG 14 antibody PIG14 antibody PPROM antibody Proliferation inducing gene 14 antibody Proliferation inducing gene 14 protein antibody RA A47 antibody RA-A47 antibody Rheumatoid arthritis antigen A 47 antibody rheumatoid arthritis antigen A-47 antibody Rheumatoid arthritis related antigen RA A47 antibody Rheumatoid arthritis-related antigen RA-A47 antibody serine (or cysteine) proteinase inhibitor clade H (heat shock protein 47) member 1 (collagen binding protein 1) antibody serine (or cysteine) proteinase inhibitor clade H (heat shock protein 47) member 2 (collagen-binding protein 2) antibody Serine or cysteine proteinase inhibitor clade H member 1 antibody Serine or cysteine proteinase inhibitor clade H member 2 antibody SERPH_HUMAN antibody Serpin H1 antibody Serpin peptidase inhibitor clade H member 1 antibody serpin peptidase inhibitor clade H (heat shock protein 47) member 1 (collagen binding protein 1) antibody Serpin peptidase inhibitor clade H member 1 antibody SERPINH1 antibody SERPINH2 antibody |
|---|---|
| Molecular Weight(MW) | 46 kDa. |
| Cellular Localization | Endoplasmic reticulum. |
WB
Western blot analysis of HSP47 on different lysates with Rabbit anti-HSP47 antibody at 1/1,000 dilution. Lane 1: HeLa cell lysate, Lane 2: NIH/3T3 cell lysate, Lane 3: C2C12 cell lysate, Lysates/proteins at 20 µg/Lane. Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-HSP47 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/800) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.| Application Notes | WB:1:500-1:2000 IHC:1:100-1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
