Application
Fig1: Western blot analysis on different lysates using anti-CLOCK rabbit polyclonal antibody. Positive control: Lane 1: PC-12 Lane 2: NIH/3T3 Lane 3: Hela Lane 4: MCF-7 Lane 5: Mouse brain Lane 6: Mouse kidneyApplication
Fig2: Immunocytochemical staining of PC-3M cells using anti-CLOCK rabbit polyclonal antibody.Application
Fig3: Immunocytochemical staining of SW480 cells using anti-CLOCK rabbit polyclonal antibody.Application
Fig4: Immunocytochemical staining of Hela cells using anti-CLOCK rabbit polyclonal antibody.Application
Fig5: Immunocytochemical staining of 293 cells using anti-CLOCK rabbit polyclonal antibody.Application
Fig6: Immunohistochemical analysis of paraffin- embedded human kidney tissue using anti-CLOCK rabbit polyclonal antibody.Application
Fig7: Immunohistochemical analysis of paraffin- embedded mouse testis tissue using anti-CLOCK rabbit polyclonal antibody.Application
Fig8: Immunohistochemical analysis of paraffin- embedded mouse skeletal muscle tissue using anti-CLOCK rabbit polyclonal antibody.Application
Fig9: Immunohistochemical analysis of paraffin- embedded human colon cancer tissue using anti-CLOCK rabbit polyclonal antibody.Application
Fig10: Flow cytometric analysis of Hela cells with CLOCK antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti rabbit IgG (FITC) was used as the secondary antibody.| Product Name | CLOCK |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | bHLHe8 antibody Circadian locomoter output cycles kaput protein antibody Circadian locomoter output cycles protein kaput antibody Circadian Locomotor Output Cycles Kaput antibody Circadium Locomotor Output Cycles Kaput antibody Class E basic helix-loop-helix protein 8 antibody CLOCK antibody Clock circadian regulator antibody Clock homolog antibody Clock protein antibody CLOCK_HUMAN antibody hCLOCK antibody KIAA0334 antibody |
| Product description | Biological timepieces called circadian clocks are responsible for the regulation of hormonal rhythms, sleep cycles and other behaviors. The superchiasmatic nucleus (SCN), which is located in the brain, was the first mammalian circadian clock to be discovered. Clock, a member of the basic-helix-loop-helix-PAS (bHLH-PAS) family of transcription factors, has also been identified as having circadian function. Mutations within the Clock gene have been shown to increase the length of the endogenous period and to cause a loss of rhythmicity of circadian oscillations. Clock contains a DNA-binding domain, a protein dimerization domain and a glutamine-rich C-terminal region, which indicates transactivation capabilities. It has been speculated that Clock may regulate circadian rhythmicity in combination with other proteins such as Per. Per is also a PAS-domain containing protein that exhibits circadian function. Highest expression of Clock is seen in the hypothalamus and the eye. |
| Immunogen | peptide |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBICCIHCFC |
| WB:1:1,000-1:5,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | bHLHe8 antibody Circadian locomoter output cycles kaput protein antibody Circadian locomoter output cycles protein kaput antibody Circadian Locomotor Output Cycles Kaput antibody Circadium Locomotor Output Cycles Kaput antibody Class E basic helix-loop-helix protein 8 antibody CLOCK antibody Clock circadian regulator antibody Clock homolog antibody Clock protein antibody CLOCK_HUMAN antibody hCLOCK antibody KIAA0334 antibody |
|---|---|
| Molecular Weight(MW) | 95kDa |
| Cellular Localization | Cytoplasm, Nucleus |
| SwissProt ID | O15516 |
|---|
Application
Fig1: Western blot analysis on different lysates using anti-CLOCK rabbit polyclonal antibody. Positive control: Lane 1: PC-12 Lane 2: NIH/3T3 Lane 3: Hela Lane 4: MCF-7 Lane 5: Mouse brain Lane 6: Mouse kidney
Application
Fig2: Immunocytochemical staining of PC-3M cells using anti-CLOCK rabbit polyclonal antibody.
Application
Fig3: Immunocytochemical staining of SW480 cells using anti-CLOCK rabbit polyclonal antibody.
Application
Fig4: Immunocytochemical staining of Hela cells using anti-CLOCK rabbit polyclonal antibody.
Application
Fig5: Immunocytochemical staining of 293 cells using anti-CLOCK rabbit polyclonal antibody.
Application
Fig6: Immunohistochemical analysis of paraffin- embedded human kidney tissue using anti-CLOCK rabbit polyclonal antibody.
Application
Fig7: Immunohistochemical analysis of paraffin- embedded mouse testis tissue using anti-CLOCK rabbit polyclonal antibody.
Application
Fig8: Immunohistochemical analysis of paraffin- embedded mouse skeletal muscle tissue using anti-CLOCK rabbit polyclonal antibody.
Application
Fig9: Immunohistochemical analysis of paraffin- embedded human colon cancer tissue using anti-CLOCK rabbit polyclonal antibody.
Application
Fig10: Flow cytometric analysis of Hela cells with CLOCK antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti rabbit IgG (FITC) was used as the secondary antibody.| Positive Control | Hela, 293, PC-12, NIH/3T3, SW480, PC-3M, human kidney tissue, mouse testis tissue, human colon cancer tissue, mouse skeletal muscle tissue. |
|---|---|
| Application Notes | WB:1:1,000-1:5,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
