WB
Western blot analysis of Myosin light chain kinase / MLCK on human kidney tissue lysates with Rabbit anti-Myosin light chain kinase / MLCK antibody at 1/1,000 dilution. Lysates/proteins at 30 µg/Lane. Exposure time: 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.IHC
Immunohistochemical analysis of paraffin- embedded mouse colon tissue using anti-MYLK rabbit polyclonal antibody.IHC
Immunohistochemical analysis of paraffin- embedded mouse smooth muscle tissue using anti-MYLK rabbit polyclonal antibody.ICC/IF
Immunocytochemical staining of MCF-7 cells using anti-MYLK rabbit polyclonal antibody.ICC/IF
Immunocytochemical staining of SW480 cells using anti-MYLK rabbit polyclonal antibody.| Product Name | Myosin light chain kinase / MLCK Rabbit Polyclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | The Ca2+/calmodulin-dependent protein kinases (CaM kinases) are a structurally related subfamily of serine/threonine kinases that includes CaMKI, CaMKII, CaMKIV and myosin light chain kinases (MYLK, also designated MLCK). The MYLK kinases phosphorylate myosin regulatory light chains to catalyze myosin interaction with actin filaments resulting in contractile activity. Non-muscle, smooth muscle and skeletal/cardiac muscle MYLK isoforms exist. The MYLK gene (also designated MYLK1) encodes both smooth muscle and non-muscle isoforms as well as telokin, a small C-terminal isoform expressed only in smooth muscle with the capacity to stabilize unphosphorylated myosin filaments. Multiple transcript variants are described for the MYLK gene. Smooth-muscle and non-muscle MYLK isoforms are expressed in a wide variety of adult and fetal tissues. The skeletal/cardiac muscle isoforms of MYLK are encoded by a separate gene, MYLK2 (also designated skMLCK). MYLK appears to be a target for PAKs (p21-activated kinases). PAK1 interaction with MYLK results in a decrease in MYLK activity and myosin light chain phosphorylation. |
| Immunogen | Recombinant protein with Human MLCK aa 230-470 / 1,914. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:1000 IHC:1:50-1:200 ICC/IF:1:50-1:200 |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | caMLCK antibody cardiac MLCK antibody Cardiac MyBP C associated Ca/CaM kinase antibody Cardiac-MyBP-C-associated Ca/CaM kinase antibody MLC kinase antibody MLCK antibody MLCK2 antibody Mylk3 antibody MYLK3_HUMAN antibody Myosin light chain kinase 3 antibody Putative myosin light chain kinase 3 antibody |
|---|---|
| Molecular Weight(MW) | 211 kDa(Observed band size: 120 kDa) |
| Cellular Localization | Cytoplasm, Cell projection, Cleavage furrow |
| SwissProt ID | Q15746 |
|---|
WB
Western blot analysis of Myosin light chain kinase / MLCK on human kidney tissue lysates with Rabbit anti-Myosin light chain kinase / MLCK antibody at 1/1,000 dilution. Lysates/proteins at 30 µg/Lane. Exposure time: 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin- embedded mouse colon tissue using anti-MYLK rabbit polyclonal antibody.
IHC
Immunohistochemical analysis of paraffin- embedded mouse smooth muscle tissue using anti-MYLK rabbit polyclonal antibody.
ICC/IF
Immunocytochemical staining of MCF-7 cells using anti-MYLK rabbit polyclonal antibody.
ICC/IF
Immunocytochemical staining of SW480 cells using anti-MYLK rabbit polyclonal antibody.| Application Notes | WB:1:1000 IHC:1:50-1:200 ICC/IF:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
