Application
Fig1: Western blot analysis of Cathepsin D on MCF-7 cell lysate using anti-Cathepsin D antibody at 1/1,000 dilution.Application
Fig2: ICC staining Cathepsin D in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining Cathepsin D in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: ICC staining Cathepsin D in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human lung tissue using anti-Cathepsin D antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Cathepsin D antibody. Counter stained with hematoxylin.Application
Fig7: Flow cytometric analysis of HepG2 cells with Cathepsin D antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).Application
Fig8: Flow cytometric analysis of MCF-7 cells with Cathepsin D antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).| Product Name | Cathepsin D |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CatD antibody CATD_HUMAN antibody Cathepsin D antibody Cathepsin D heavy chain antibody CD antibody Ceroid lipofuscinosis neuronal 10 antibody CLN10 antibody CPSD antibody ctsd antibody Epididymis secretory sperm binding protein Li 130P antibody HEL S 130P antibody Lysosomal aspartyl peptidase antibody Lysosomal aspartyl protease antibody MGC2311 antibody |
| Product description | The cathepsin family of proteolytic enzymes contains several diverse classes of proteases. The cysteine protease class comprises cathepsins B, L, H, K, S, and O. The aspartyl protease class is composed of cathepsins D and E. Cathepsin G is in the serine protease class. Most cathepsins are lysosomal and each is involved in cellular metabolism, participating in various events such as peptide biosynthesis and protein degradation. Cathepsins may also cleave some protein precursors, thereby releasing regulatory peptides. The promoter region of the cathepsin D gene contains five Sp1 binding sites and four AP-2 binding sites. |
| Immunogen | Peptide |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBICCIHCFC |
| WB:1:500 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:200 |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Alternative Names | CatD antibody CATD_HUMAN antibody Cathepsin D antibody Cathepsin D heavy chain antibody CD antibody Ceroid lipofuscinosis neuronal 10 antibody CLN10 antibody CPSD antibody ctsd antibody Epididymis secretory sperm binding protein Li 130P antibody HEL S 130P antibody Lysosomal aspartyl peptidase antibody Lysosomal aspartyl protease antibody MGC2311 antibody |
|---|---|
| Molecular Weight(MW) | 27 kDa |
| Cellular Localization | Lysosome. Melanosome. Secreted, extracellular space. |
Application
Fig1: Western blot analysis of Cathepsin D on MCF-7 cell lysate using anti-Cathepsin D antibody at 1/1,000 dilution.
Application
Fig2: ICC staining Cathepsin D in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining Cathepsin D in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: ICC staining Cathepsin D in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human lung tissue using anti-Cathepsin D antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Cathepsin D antibody. Counter stained with hematoxylin.
Application
Fig7: Flow cytometric analysis of HepG2 cells with Cathepsin D antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).
Application
Fig8: Flow cytometric analysis of MCF-7 cells with Cathepsin D antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).| Positive Control | MCF-7, Hela, HepG2, human liver tissue, human lung tissue. |
|---|---|
| Application Notes | WB:1:500 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 0.5%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide. |
