Application
Fig1: Western blot analysis of HDAC2 on different cell lysates using anti-HDAC2 antibody at 1/1,000 dilution. Positive control： Lane1: SH-SY5Y Lane2: 293T Lane3: Hela Lane4: PC-12Application
Fig2: ICC staining HDAC2 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining HDAC2 in NIH-3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: ICC staining HDAC2 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-HDAC2 antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-HDAC2 antibody. Counter stained with hematoxylin.Application
Fig7: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-HDAC2 antibody. Counter stained with hematoxylin.Application
Fig8: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-HDAC2 antibody. Counter stained with hematoxylin.Application
Fig9: Flow cytometric analysis of SH-SY5Y cells with HDAC2 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).| Product Name | Histone Deacetylase 2 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | D10Wsu179e antibody HD 2 antibody HD2 antibody HDAC 2 antibody Hdac2 antibody HDAC2_HUMAN antibody Histone deacetylase 2 (HD2) antibody Histone deacetylase 2 antibody OTTHUMP00000017046 antibody OTTHUMP00000227077 antibody OTTHUMP00000227078 antibody RPD3 antibody transcriptional regulator homolog RPD3 antibody YAF1 antibody YY1 associated factor 1 antibody YY1 transcription factor binding protein antibody Yy1bp antibody |
| Product description | In the intact cell, DNA closely associates with histones and other nuclear proteins to form chromatin. The remodeling of chromatin is believed to be a critical component of transcriptional regulation, and a major source of this remodeling is brought about by the acetylation of nucleosomal histones. Acetylation of lysine residues in the amino terminal tail domain of histone results in an allosteric change in the nucleosomal conformation and an increased accessibility to transcription factors by DNA. Conversely, the deacetylation of histones is associated with transcriptional silencing. Several mammalian proteins have been identified as nuclear histone acetylases, including GCN5, PCAF (for p300/CBP-associated factor), p300/CBP and the TFIID subunit TAF II p250. Mammalian HDAC1 (also designated HD1) and HDAC2 (also designated mammalian RPD3), both of which are related to the yeast transcriptional regulator Rpd3p, have been identified as histone deacetylases. |
| Immunogen | Recombinant protein. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBICCIHCFC |
| WB:1:500-1:1,000 ICC:1:500-1:2,000 IHC:1:100-1:200 FC:1:50-1:100 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | D10Wsu179e antibody HD 2 antibody HD2 antibody HDAC 2 antibody Hdac2 antibody HDAC2_HUMAN antibody Histone deacetylase 2 (HD2) antibody Histone deacetylase 2 antibody OTTHUMP00000017046 antibody OTTHUMP00000227077 antibody OTTHUMP00000227078 antibody RPD3 antibody transcriptional regulator homolog RPD3 antibody YAF1 antibody YY1 associated factor 1 antibody YY1 transcription factor binding protein antibody Yy1bp antibody |
|---|---|
| Molecular Weight(MW) | 55 kDa |
| Cellular Localization | Nucleus. Cytoplasm. |
Application
Fig1: Western blot analysis of HDAC2 on different cell lysates using anti-HDAC2 antibody at 1/1,000 dilution. Positive control： Lane1: SH-SY5Y Lane2: 293T Lane3: Hela Lane4: PC-12
Application
Fig2: ICC staining HDAC2 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining HDAC2 in NIH-3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: ICC staining HDAC2 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-HDAC2 antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-HDAC2 antibody. Counter stained with hematoxylin.
Application
Fig7: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-HDAC2 antibody. Counter stained with hematoxylin.
Application
Fig8: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-HDAC2 antibody. Counter stained with hematoxylin.
Application
Fig9: Flow cytometric analysis of SH-SY5Y cells with HDAC2 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).| Positive Control | SH-SY5Y, 293T, Hela, PC-12, LOVO, NIH-3T3, human tonsil tissue, human colon cancer tissue, human kidney tissue, mouse brain tissue. |
|---|---|
| Application Notes | WB:1:500-1:1,000 ICC:1:500-1:2,000 IHC:1:100-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 0.5%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide. |
