Application
Fig1: Western blot analysis of PCBP1 on different cell lysate using anti-PCBP1 antibody at 1/1,000 dilution. Positive control： Lane1: F9 Lane2: NIH-3T3 Lane3: K562Application
Fig2: ICC staining PCBP1 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining PCBP1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: ICC staining PCBP1 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig5: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-PCBP1 antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-PCBP1 antibody. Counter stained with hematoxylin.Application
Fig7: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-PCBP1 antibody. Counter stained with hematoxylin.| Product Name | PCBP1 Rabbit Polyclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Single-stranded nucleic acid binding protein that binds preferentially to oligo dC. In case of infection by poliovirus, plays a role in initiation of viral RNA replication in concert with the viral protein 3CD. Abundantly expressed in skeletal muscle, thymus and peripheral blood leukocytes while a lower expression is observed in prostate, spleen, testis, ovary, small intestine, heart, liver, adrenal and thyroid glands. |
| Immunogen | Recombinant protein within Human PCBP1 aa aa 1-162 / 356. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICCIHCWB |
| WB:1:500-1:1,000 ICC:1:200-1:1,000 IHC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Alternative Names | Alpha-CP1 antibody Heterogeneous nuclear ribonucleoprotein E1 antibody heterogenous nuclear ribonucleoprotein E1 antibody heterogenous nuclear ribonucleoprotein X antibody hnRNP E1 antibody hnRNP-E1 antibody hnRNP-X antibody HNRPE1 antibody HNRPX antibody nucleic acid binding protein sub 2.3 antibody Nucleic acid- binding protein SUB2.3 antibody Nucleic acid-binding protein SUB2.3 antibody PCBP1 antibody PCBP1_HUMAN antibody poly(rC) binding protein 1 antibody Poly(rC)-binding protein 1 antibody rCbinding protein 1 antibody |
|---|---|
| Molecular Weight(MW) | 37 kDa |
| Cellular Localization | Nucleus. Cytoplasm. |
Application
Fig1: Western blot analysis of PCBP1 on different cell lysate using anti-PCBP1 antibody at 1/1,000 dilution. Positive control： Lane1: F9 Lane2: NIH-3T3 Lane3: K562
Application
Fig2: ICC staining PCBP1 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining PCBP1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: ICC staining PCBP1 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-PCBP1 antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-PCBP1 antibody. Counter stained with hematoxylin.
Application
Fig7: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-PCBP1 antibody. Counter stained with hematoxylin.| Positive Control | F9, K562, NIH-3T3, A549, HepG2, MCF-7, human spleen tissue, mouse testis tissue, rat brain tissue. |
|---|---|
| Application Notes | WB:1:500-1:1,000 ICC:1:200-1:1,000 IHC:1:50-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 0.5%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide. |
