Application
Fig1: Western blot analysis of IKK beta on different lysates using anti-IKK beta antibody at 1/1,000 dilution. Positive control： Lane1: HelaLane2: 293TLane3: Mouse spleen tissueApplication
Fig2: ICC staining IKK beta in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining IKK beta in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-IKK beta antibody. Counter stained with hematoxylin.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-IKK beta antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-IKK beta antibody. Counter stained with hematoxylin.Application
Fig7: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-IKK beta antibody. Counter stained with hematoxylin.Application
Fig8: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-IKK beta antibody. Counter stained with hematoxylin.Application
Fig9: Flow cytometric analysis of A549 cells with IKK beta antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).| Product Name | IKK beta |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | I kappa B kinase 2 antibody I kappa B kinase beta antibody I-kappa-B kinase 2 antibody I-kappa-B-kinase beta antibody IkBKB antibody IKK beta antibody IKK-B antibody IKK-beta antibody IKK2 antibody IKKB antibody IKKB_HUMAN antibody IMD15 antibody Inhibitor of kappa light polypeptide gene enhancer in B cells, kinase beta antibody Inhibitor of nuclear factor kappa-B kinase subunit beta antibody NFKBIKB antibody Nuclear factor NF-kappa-B inhibitor kinase beta antibody |
| Product description | The transcription factor NFkB is retained in the cytoplasm in an inactive form by the inhibitory protein IkB. Activation of NFkB requires that IkB be phosphorylated on specific serine residues, which results in targeted degradation of IkB. IkB kinase α (IKKα), previously designated CHUK, interacts with IkB-α and specifically phosphorylates Iκβ-α on Serine 32 and 36, the sites that trigger its degradation. IKKα appears to be critical for NFkB activation in response to proinflammatory cytokines. Phosphorylation of IkB by IKKα is stimulated by the NFkB inducing kinase (NIK), which itself is a central regulator for NFkB activation in response to TNF and IL-1. The functional IKK complex contains three subunits, IKKα, IKKβ and IKKγ (also designated NEMO), and each appear to make essential contributions to IkB phosphorylation. |
| Immunogen | Recombinant protein |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBICCIHCFC |
| WB:1:1,000-1:5,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | I kappa B kinase 2 antibody
I kappa B kinase beta antibody
I-kappa-B kinase 2 antibody
I-kappa-B-kinase beta antibody
IkBKB antibody
IKK beta antibody
IKK-B antibody
IKK-beta antibody
IKK2 antibody
IKKB antibody
IKKB_HUMAN antibody
IMD15 antibody
Inhibitor of kappa light polypeptide gene enhancer in B cells kinase beta antibody Inhibitor of nuclear factor kappa-B kinase subunit beta antibody NFKBIKB antibody Nuclear factor NF-kappa-B inhibitor kinase beta antibody |
|---|---|
| Molecular Weight(MW) | 87/79 kDa |
| Cellular Localization | Nucleus. Cytoplasm. |
Application
Fig1: Western blot analysis of IKK beta on different lysates using anti-IKK beta antibody at 1/1,000 dilution. Positive control： Lane1: HelaLane2: 293TLane3: Mouse spleen tissue
Application
Fig2: ICC staining IKK beta in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining IKK beta in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-IKK beta antibody. Counter stained with hematoxylin.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-IKK beta antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-IKK beta antibody. Counter stained with hematoxylin.
Application
Fig7: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-IKK beta antibody. Counter stained with hematoxylin.
Application
Fig8: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-IKK beta antibody. Counter stained with hematoxylin.
Application
Fig9: Flow cytometric analysis of A549 cells with IKK beta antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).| Positive Control | Hela, 293T, mouse spleen tissue lysate, HUVEC, rat testis tissue, human tonsil tissue, human breast tissue, human placenta tissue, mouse testis tissue. |
|---|---|
| Application Notes | WB:1:1,000-1:5,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃after thawing. Aliquot store at -20℃or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 0.5%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide. |
