Application
Fig1: Western blot analysis of NSUN4 on different cell lysates using anti-NSUN4 antibody at 1/1,000 dilution. Positive control： Lane1: MCF-7 Lane2: 293Application
Fig2: ICC staining NSUN4 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining NSUN4 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: ICC staining NSUN4 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-NSUN4 antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-NSUN4 antibody. Counter stained with hematoxylin.Application
Fig7: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-NSUN4 antibody. Counter stained with hematoxylin.| Product Name | NSUN4 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | 5 methylcytosine rRNA methyltransferase NSUN4 antibody EC=2.1.1.- antibody NOL1/NOP2/Sun domain family member 4 antibody NOL1/NOP2/Sun domain family, member 4 antibody NOP2/Sun domain family, member 4 antibody Nsun4 antibody NSUN4_HUMAN antibody Putative methyltransferase NSUN4 antibody RP4-603I14.2 antibody Shtap antibody Sperm head and tail associated protein antibody |
| Product description | Involved in mitochondrial ribosome assembly. 5-methylcytosine rRNA methyltransferase that probably is involved in mitochondrial ribosome small subunit (SSU) maturation by methylation of mitochondrial 12S rRNA; the function is independent of MTERFD2/MTERF4 and assembled mitochondrial ribosome large subunit (LSU). Targeted to LSU by MTERFD2/MTERF4 and probably is involved in a final step in ribosome biogenesis to ensure that SSU and LSU are assembled. In vitro can methylate 16S rRNA of the LSU; the methylation is enhanced by MTERFD/MTERF4. |
| Immunogen | Recombinant protein |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBICCIHC |
| WB:1:500-1:1,000 ICC:1:50-1:200 IHC:1:50-1:200 |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Alternative Names | 5 methylcytosine rRNA methyltransferase NSUN4 antibody
EC=2.1.1.- antibody
NOL1/NOP2/Sun domain family member 4 antibody
NOL1/NOP2/Sun domain family member 4 antibody NOP2/Sun domain family member 4 antibody Nsun4 antibody NSUN4_HUMAN antibody Putative methyltransferase NSUN4 antibody RP4-603I14.2 antibody Shtap antibody Sperm head and tail associated protein antibody |
|---|---|
| Molecular Weight(MW) | 43 kDa |
| Cellular Localization | Mitochondrion. |
| SwissProt ID | Q96CB9 |
|---|
Application
Fig1: Western blot analysis of NSUN4 on different cell lysates using anti-NSUN4 antibody at 1/1,000 dilution. Positive control： Lane1: MCF-7 Lane2: 293
Application
Fig2: ICC staining NSUN4 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining NSUN4 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: ICC staining NSUN4 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-NSUN4 antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-NSUN4 antibody. Counter stained with hematoxylin.
Application
Fig7: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-NSUN4 antibody. Counter stained with hematoxylin.| Positive Control | MCF-7, 293, A549, HepG2, human liver cancer tissue, human kidney tissue, mouse testis tissue. |
|---|---|
| Application Notes | WB:1:500-1:1,000 ICC:1:50-1:200 IHC:1:50-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 0.5%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide. |
