Application
Fig1: Western blot analysis of Neurofilament on mouse brain tissue lysate using anti-Neurofilament antibody at 1/500 dilution.Application
Fig2: ICC staining Neurofilament in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-Neurofilament antibody. Counter stained with hematoxylin.Application
Fig4: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Neurofilament antibody. Counter stained with hematoxylin.| Product Name | Neurofilament |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | 150kDa medium antibody 160 kDa neurofilament protein antibody 2 kDa neurofilament protein antibody200 kDa neurofilament protein antibody 68 kDa neurofilament protein antibody AI847934 antibody CMT1F antibody CMT2CC antibody CMT2E antibody FLJ53642 antibody KIAA0845 antibody light molecular weight neurofilament protein antibody Micro glutamic acid-rich protein antibody mKIAA0845 antibody Nef3 antibody NEFH antibody NEFL antibody NEFM antibody Neurofilament 3 antibody Neurofilament heavy polypeptide 200kDa antibody Neurofilament heavy polypeptide antibody Neurofilament light polypeptide 68kDa antibody Neurofilament light polypeptide antibody Neurofilament medium polypeptide 150kDa antibody Neurofilament medium polypeptide antibody Neurofilament protein heavy polypeptide antibody Neurofilament protein light chain antibody Neurofilament protein light polypeptide antibody Neurofilament protein medium polypeptide antibody neurofilament subunit NF-L antibody Neurofilament triplet H protein antibody Neurofilament triplet L protein antibody Neurofilament triplet M protein antibody neurofilament-3 (150 kD medium) antibody NF-H antibody NF-L antibody NF-M antibody NF160 antibody NF165 antibody NF200 antibody NF68 antibody NFH antibody NFL antibody NFM antibody NFM_HUMAN antibody PPP1R110 antibody protein phosphatase 1, regulatory subunit 110 antibody |
| Product description | Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. NF-H has an important function in mature axons that is not subserved by the two smaller NF proteins. Neurofilament-H (NF-H), also known as neurofilament heavy polypeptide, and Neurofilament-L (NF-L), also known as neurofilament light polypeptide, members of the intermediate filament family, are major components of neuronal cytoskeletons. Neurofilaments are dynamic structures; they contain phosphorylation sites for a large number of protein kinases, including protein kinase A, protein kinase C, cyclin-dependent kinase 5, extracellular signal regulated kinase, glycogen synthase kinase-3, and stress-activated protein kinase gamma. In addition to their role in the control of axon caliber, neurofilaments may affect other cytoskeletal elements, such as microtubules and Actin filaments. Changes in neurofilament phosphorylation or metabolism are frequently observed in neurodegenerative diseases, including amyotrophic lateral sclerosis (ALS), Parkinson's disease and Alzheimer's disease. |
| Immunogen | Recombinant protein |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBICCIHC |
| WB:1:500-1:1000 ICC:1:50-1:200 IHC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | 150kDa medium antibody
160 kDa neurofilament protein antibody
2 kDa neurofilament protein antibody200 kDa neurofilament protein antibody
68 kDa neurofilament protein antibody
AI847934 antibody
CMT1F antibody
CMT2CC antibody
CMT2E antibody
FLJ53642 antibody
KIAA0845 antibody
light molecular weight neurofilament protein antibody
Micro glutamic acid-rich protein antibody
mKIAA0845 antibody
Nef3 antibody
NEFH antibody
NEFL antibody
NEFM antibody
Neurofilament 3 antibody
Neurofilament heavy polypeptide 200kDa antibody
Neurofilament heavy polypeptide antibody
Neurofilament light polypeptide 68kDa antibody
Neurofilament light polypeptide antibody
Neurofilament medium polypeptide 150kDa antibody
Neurofilament medium polypeptide antibody
Neurofilament protein heavy polypeptide antibody
Neurofilament protein light chain antibody
Neurofilament protein light polypeptide antibody
Neurofilament protein medium polypeptide antibody
neurofilament subunit NF-L antibody
Neurofilament triplet H protein antibody
Neurofilament triplet L protein antibody
Neurofilament triplet M protein antibody
neurofilament-3 (150 kD medium) antibody
NF-H antibody
NF-L antibody
NF-M antibody
NF160 antibody
NF165 antibody
NF200 antibody
NF68 antibody
NFH antibody
NFL antibody
NFM antibody
NFM_HUMAN antibody
PPP1R110 antibody
protein phosphatase 1 regulatory subunit 110 antibody |
|---|---|
| Molecular Weight(MW) | 200 kDa |
| Cellular Localization | Cytoplasm, Intermediate filament. |
Application
Fig1: Western blot analysis of Neurofilament on mouse brain tissue lysate using anti-Neurofilament antibody at 1/500 dilution.
Application
Fig2: ICC staining Neurofilament in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-Neurofilament antibody. Counter stained with hematoxylin.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Neurofilament antibody. Counter stained with hematoxylin.| Positive Control | N2A, rat brain tissue, mouse brain tissue. |
|---|---|
| Application Notes | WB:1:500-1:1000 ICC:1:50-1:200 IHC:1:50-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃after thawing. Aliquot store at -20℃or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 0.5%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide. |
