Application
Fig1: Western blot analysis of DDB1 on different cell lysate using anti-DDB1 antibody at 1/1,000 dilution. Positive control： Lane1: Mouse colon tissue Lane2: PC-12 Lane3: SihaApplication
Fig2: ICC staining DDB1 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining DDB1 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: ICC staining DDB1 in SK-Br-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig5: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-DDB1 antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-DDB1 antibody. Counter stained with hematoxylin.Application
Fig7: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-DDB1 antibody. Counter stained with hematoxylin.Application
Fig8: Flow cytometric analysis of K562 cells with DDB1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).| Product Name | DDB1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Damage specific DNA binding protein 1 antibody Damage-specific DNA-binding protein 1 antibody DDB 1 antibody DDB p127 subunit antibody Ddb1 antibody DDB1_HUMAN antibody DDBa antibody DNA damage binding protein 1 antibody DNA damage-binding protein 1 antibody DNA damage-binding protein a antibody HBV X-associated protein 1 antibody UV damaged DNA binding factor antibody UV damaged DNA binding protein 1 antibody UV DDB 1 antibody UV DDB1 antibody UV-damaged DNA-binding factor antibody UV-damaged DNA-binding protein 1 antibody UV-DDB 1 antibody X associated protein 1 antibody XAP 1 antibody XAP-1 antibody XAP1 antibody Xeroderma pigmentosum group E complementing protein antibody Xeroderma pigmentosum group E-complementing protein antibody XPCe antibody XPE antibody XPE BF antibody XPE binding factor antibody XPE-BF antibody XPE-binding factor antibody |
| Product description | Damaged DNA binding protein (DDB) is a heterodimer composed of two subunits, p127 and p48, which are designated DDB1 and DDB2, respectively. The DDB heterodimer is involved in repairing DNA damaged by ultraviolet light. Specifically, DDB, also designated UV-damaged DNA binding protein (UV-DDB), xeroderma pigmentosum group E binding factor (XPE-BF) and hepatitis B virus X-associated protein 1 (XAP-1), binds to damaged cyclobutane pyrimidine dimers (CPDs). Mutations in the DDB2 gene are implicated as causes of xeroderma pigmentosum group E, an autosomal recessive disease in which patients are defective in nucleotide excision DNA repair. XPE is characterized by hypersensitivity of the skin to sunlight with a high frequency of skin cancer as well as neurologic abnormalities. The hepatitis B virus (HBV) X protein interacts with DDB1, which may mediate HBx transactivation. |
| Immunogen | Recombinant protein |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBICCIHCFC |
| WB:1:500-1:1000 ICC:1:100-1:500 IHC:1:100-1:500 FC:1:50-1:100 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | Damage specific DNA binding protein 1 antibody Damage-specific DNA-binding protein 1 antibody DDB 1 antibody DDB p127 subunit antibody Ddb1 antibody DDB1_HUMAN antibody DDBa antibody DNA damage binding protein 1 antibody DNA damage-binding protein 1 antibody DNA damage-binding protein a antibody HBV X-associated protein 1 antibody UV damaged DNA binding factor antibody UV damaged DNA binding protein 1 antibody UV DDB 1 antibody UV DDB1 antibody UV-damaged DNA-binding factor antibody UV-damaged DNA-binding protein 1 antibody UV-DDB 1 antibody X associated protein 1 antibody XAP 1 antibody XAP-1 antibody XAP1 antibody Xeroderma pigmentosum group E complementing protein antibody Xeroderma pigmentosum group E-complementing protein antibody XPCe antibody XPE antibody XPE BF antibody XPE binding factor antibody XPE-BF antibody XPE-binding factor antibody |
|---|---|
| Molecular Weight(MW) | 127 kDa, additional band 150kDa |
| Cellular Localization | Nucleus. Cytoplasm. |
Application
Fig1: Western blot analysis of DDB1 on different cell lysate using anti-DDB1 antibody at 1/1,000 dilution. Positive control： Lane1: Mouse colon tissue Lane2: PC-12 Lane3: Siha
Application
Fig2: ICC staining DDB1 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining DDB1 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: ICC staining DDB1 in SK-Br-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-DDB1 antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-DDB1 antibody. Counter stained with hematoxylin.
Application
Fig7: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-DDB1 antibody. Counter stained with hematoxylin.
Application
Fig8: Flow cytometric analysis of K562 cells with DDB1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).| Positive Control | Mouse colon tissue lysate, PC-12, Siha, A549, SH-SY5Y, SK-Br-3, K562, human breast, human kidney, rat brain. |
|---|---|
| Application Notes | WB:1:500-1:1000 ICC:1:100-1:500 IHC:1:100-1:500 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃ Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 0.5%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide. |
