Application
Fig1: Western blot analysis of Tyrosinase on B16F1 cell lysates using anti- Tyrosinase antibody at 1/1,000 dilution.Application
Fig2: ICC staining Tyrosinase in B16F1 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining Tyrosinase in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: Immunohistochemical analysis of paraffin-embedded human urethral melanoma tissue using anti-Tyrosinase antibody. Counter stained with hematoxylin.Application
Fig5: Flow cytometric analysis of A431 cells with Tyrosinase antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Product Name | Tyrosinase |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ATN antibody CMM8 antibody LB24 AB antibody LB24-AB antibody Monophenol monooxygenase antibody OCA1 antibody OCA1A antibody OCAIA antibody Oculocutaneous albinism IA antibody SHEP3 antibody SK29 AB antibody SK29-AB antibody Tumor rejection antigen AB antibody TYR antibody TYRO_HUMAN antibody tyrosinase (oculocutaneous albinism IA) antibody Tyrosinase antibody |
| Product description | This is a copper-containing oxidase that functions in the formation of pigments such as melanins and other polyphenolic compounds. Catalyzes the initial and rate limiting step in the cascade of reactions leading to melanin production from tyrosine. In addition to hydroxylating tyrosine to DOPA (3,4-dihydroxyphenylalanine), also catalyzes the oxidation of DOPA to DOPA-quinone, and possibly the oxidation of DHI (5,6-dihydroxyindole) to indole-5,6 quinone. |
| Immunogen | Peptide |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBICCIHCFC |
| WB:1:200-1:500 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:200: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Alternative Names | ATN antibody CMM8 antibody LB24 AB antibody LB24-AB antibody Monophenol monooxygenase antibody OCA1 antibody OCA1A antibody OCAIA antibody Oculocutaneous albinism IA antibody SHEP3 antibody SK29 AB antibody SK29-AB antibody Tumor rejection antigen AB antibody TYR antibody TYRO_HUMAN antibody tyrosinase (oculocutaneous albinism IA) antibody Tyrosinase antibody |
|---|---|
| Molecular Weight(MW) | 70 kDa |
| Cellular Localization | Membrane. |
| SwissProt ID | P14679 |
|---|
Application
Fig1: Western blot analysis of Tyrosinase on B16F1 cell lysates using anti- Tyrosinase antibody at 1/1,000 dilution.
Application
Fig2: ICC staining Tyrosinase in B16F1 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining Tyrosinase in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded human urethral melanoma tissue using anti-Tyrosinase antibody. Counter stained with hematoxylin.
Application
Fig5: Flow cytometric analysis of A431 cells with Tyrosinase antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Positive Control | B16F1, Hela, human urethral melanoma tissue, A431. |
|---|---|
| Application Notes | WB:1:200-1:500 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:200: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 0.5%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide. |
