Application
Fig1: ICC staining Retrovirus-related Gag in Retrovirus-related Gag transfected 293 cells (red) and HA-tag staining (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.| Product Name | Retrovirus-related Gag |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Intracisternal A-type particles (IAP) are defective endogenous retroviruses that accumulate in the endoplasmic reticulum (ER) of rodent cells. The enveloped particles are produced by assembly and budding of IAP Gag polyproteins at the ER membrane. Readthrough of the terminator UAA occurs between the codons for Met-218 and Ala-219. This particle is a defective retrovirus. |
| Immunogen | Peptide |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC |
| ICC:1:50-1:200 |
|
| Species Reactivity | Mouse |
| Concentration | 1mg/ml |
| Molecular Weight(MW) | 29 kDa |
|---|---|
| Cellular Localization | Viral capsid. |
| SwissProt ID | P31790 |
|---|
Application
Fig1: ICC staining Retrovirus-related Gag in Retrovirus-related Gag transfected 293 cells (red) and HA-tag staining (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.| Positive Control | Retrovirus-related Gag protein transfected 293 cells. |
|---|---|
| Application Notes | ICC:1:50-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 0.5%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide. |
