Application
Fig1: Western blot analysis on HeLa cell lysate using anti- alpha-Tubulin.Application
Fig2： Immunohistochemical analysis of paraffin-embedded human gastric carcinoma using anti- alpha-Tubulin.| Product Name | Alpha Tubulin [6J8] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Alpha tubulin ubiquitous antibody Alpha-tubulin ubiquitous antibody K alpha 1 antibody TBA1B_HUMAN antibody TUBA1B antibody Tubulin alpha 1B antibody Tubulin alpha 1B chain antibody Tubulin alpha ubiquitous antibody Tubulin alpha ubiquitous chain antibody Tubulin alpha-1B chain antibody Tubulin alpha-ubiquitous chain antibody Tubulin K alpha 1 antibody Tubulin K-alpha-1 antibody |
| Product description | Microtubules are essential parts in eukaryotic cell structures, transportation, and mitosis. It consists mainly of 2 soluble protein subunits, alpha and beta tubulin. Beta tubulin binds to alpha tubulin to form tubulin heterodimer which is post-translationally modified (1). The tubulin dimer complex binds to GTP and assembles onto the positive ends of microtubules. After incorporation into the microtubules, bound GTP is hydrolyzed by beta tubulin. The stability of the dimer in the microtubules is depended on presence of beta tubulin, where dimer with GTP bound beta-tubulin is stable to microtubule incorporation (2). For both type of tubulin, the carboxy-terminal tail is post-translationally modified to regulate associated protein binding at microtubule surface (3). A mutation on alpha tubulin has been linked to abnormal neuronal migration and neurodevelopmental disorders in humans (4). |
| Immunogen | peptide |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBICCIHCIP |
| WB:1:1,000 ICC:1:100 IHC:1:100 IP:1:10-100 FC:1:10-100 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | Alpha tubulin ubiquitous antibody Alpha-tubulin ubiquitous antibody K alpha 1 antibody TBA1B_HUMAN antibody TUBA1B antibody Tubulin alpha 1B antibody Tubulin alpha 1B chain antibody Tubulin alpha ubiquitous antibody Tubulin alpha ubiquitous chain antibody Tubulin alpha-1B chain antibody Tubulin alpha-ubiquitous chain antibody Tubulin K alpha 1 antibody Tubulin K-alpha-1 antibody |
|---|---|
| Molecular Weight(MW) | 50 kDa |
| Cellular Localization | Cytoplasm |
| SwissProt ID | P68363 |
|---|
Application
Fig1: Western blot analysis on HeLa cell lysate using anti- alpha-Tubulin.
Application
Fig2： Immunohistochemical analysis of paraffin-embedded human gastric carcinoma using anti- alpha-Tubulin.| Positive Control | Hela |
|---|---|
| Application Notes | WB:1:1,000 ICC:1:100 IHC:1:100 IP:1:10-100 FC:1:10-100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20 ℃. Stable for 12 months from date of receipt |
| Storage Buffer | 1*TBS (pH7.4), 0.5%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
