Application
Fig1: Western blot analysis Hela cell lysate using IκB-α RabMAb.Application
Fig2： Immunohistochemical analysis of paraffin-embedded human prostate carcinoma using IκB-α RabMAb.| Product Name | IkappaB alpha [1E3] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | I kappa B alpha antibody I-kappa-B-alpha antibody IkappaBalpha antibody IkB-alpha antibody IKBA antibody IKBA_HUMAN antibody IKBalpha antibody MAD 3 antibody MAD3 antibody Major histocompatibility complex enhancer-binding protein MAD3 antibody NF kappa B inhibitor alpha antibody NF-kappa-B inhibitor alpha antibody NFKBI antibody NFKBIA antibody Nuclear factor of kappa light chain gene enhancer in B cells antibody Nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor alpha antibody |
| Product description | The NF-κB/Rel transcription factors are present in the cytosol in an inactive state, complexed with the inhibitory IκB-α protein. In response to many different NF-κB-inducing agents—including T-cell mitogens, proinflammatory cytokines, and viral transactivators—the inhibitory IκB-α is rapidly phosphorylated and degraded. Phosphorylation of IκB-α at Ser32 and Ser36 has been shown to stimulate conjugation with ubiquitin and subsequent degradation of IκB-α. Phosphorylation of IκB-α at Ser32 and Ser36 is critical for activation of NF-κB, which then translocates to the nucleus and induces transcription of genes that protect organism. |
| Immunogen | peptide |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHCICCIP |
| WB:1:1,000 ICC:1:100 IHC:1:100 IP:1:10-100 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | I kappa B alpha antibody I-kappa-B-alpha antibody IkappaBalpha antibody IkB-alpha antibody IKBA antibody IKBA_HUMAN antibody IKBalpha antibody MAD 3 antibody MAD3 antibody Major histocompatibility complex enhancer-binding protein MAD3 antibody NF kappa B inhibitor alpha antibody NF-kappa-B inhibitor alpha antibody NFKBI antibody NFKBIA antibody Nuclear factor of kappa light chain gene enhancer in B cells antibody Nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor alpha antibody |
|---|---|
| Molecular Weight(MW) | 35 kDa |
| Cellular Localization | Cytoplasm, Nucleus |
| SwissProt ID | P25963 |
|---|
Application
Fig1: Western blot analysis Hela cell lysate using IκB-α RabMAb.
Application
Fig2： Immunohistochemical analysis of paraffin-embedded human prostate carcinoma using IκB-α RabMAb.| Positive Control | Hela, human prostate carcinoma |
|---|---|
| Application Notes | WB:1:1,000 ICC:1:100 IHC:1:100 IP:1:10-100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
