Application
Fig1: Western blot analysis on (A) MCF-7, (B) Jurkat, (C) HeLa, and (D) HT-29 cell lysates using anti-MGMT.Application
Fig2： Immunohistochemical analysis of paraffin-embedded human clear cell carcinoma tissue using anti-MGMT.| Product Name | MGMT |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | 6 O methylguanine DNA methyltransferase antibody 6-O-methylguanine-DNA methyltransferase antibody Agat antibody AGT antibody AI267024 antibody EC 2.1.1.63 antibody Methylated DNA protein cysteine methyltransferase antibody Methylated-DNAprotein-cysteine methyltransferase antibody Methylguanine DNA methyltransferase antibody MGC107020 antibody MGMT antibody MGMT_HUMAN antibody O 6 methylguanine DNA alkyltransferase antibody O 6 methylguanine DNA methyltransferase antibody O-6-methylguanine-DNA methyltransferase antibody O-6-methylguanine-DNA-alkyltransferase antibody |
| Product description | O(6)-methylguanine-DNA methyltransferase (MGMT) is a DNA repair protein that protects tissues against toxic and carcinogenic effects of alkylating agents (1). It is involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA, and repairs alkylated guanine in DNA; a suicide reaction, because the enzyme is irreversibly inactivated (2). MGMT is ubiquitously expressed in normal human tissues but is overexpressed in all types of human tumors, including colon cancer, breast cancer, leukemia, lymphomas, and myeloma. Although tumor cells have many mechanisms of resistance to alkylating agents, the expression of MGMT in tumors has a protective effect against cell death induced by alkylating agents, showing a strong correlation between MGMT activity and tumor drug resistance (3). |
| Immunogen | peptide |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:1,000 IHC:1:250-500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | 6 O methylguanine DNA methyltransferase antibody 6-O-methylguanine-DNA methyltransferase antibody Agat antibody AGT antibody AI267024 antibody EC 2.1.1.63 antibody Methylated DNA protein cysteine methyltransferase antibody Methylated-DNAprotein-cysteine methyltransferase antibody Methylguanine DNA methyltransferase antibody MGC107020 antibody MGMT antibody MGMT_HUMAN antibody O 6 methylguanine DNA alkyltransferase antibody O 6 methylguanine DNA methyltransferase antibody O-6-methylguanine-DNA methyltransferase antibody O-6-methylguanine-DNA-alkyltransferase antibody |
|---|---|
| Molecular Weight(MW) | 23 kDa |
| Cellular Localization | Nucleus |
| SwissProt ID | P16455 |
|---|
Application
Fig1: Western blot analysis on (A) MCF-7, (B) Jurkat, (C) HeLa, and (D) HT-29 cell lysates using anti-MGMT.
Application
Fig2： Immunohistochemical analysis of paraffin-embedded human clear cell carcinoma tissue using anti-MGMT.| Positive Control | Hela, MCF-7, Jurkat, HT-29. |
|---|---|
| Application Notes | WB:1:1,000 IHC:1:250-500 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20 ℃. Stable for 12 months from date of receipt |
| Storage Buffer | 1*TBS (pH7.4), 0.5%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
