Application
Fig1: Western blot analysis of AQP4 expression in non-transfected (A) and mouse AQP4 transfected (B) 293T whole cell lysates and mouse kidney tissue extract (C).Application
Fig2： Immunoperoxidase staining of formalin fixed, paraffin-embedded mouse kidney tissue showing membrane localization.| Product Name | AQUAPORIN 4 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | AQP 4 antibody AQP-4 antibody AQP4 antibody AQP4_HUMAN antibody Aquaporin type 4 antibody Aquaporin-4 antibody Aquaporin4 antibody HMIWC 2 antibody HMIWC2 antibody Mercurial insensitive water channel antibody Mercurial-insensitive water channel antibody MGC22454 antibody MIWC antibody WCH 4 antibody WCH4 antibody |
| Product description | In skeletal muscle, AQP4 (aquaporin 4 also known as mercurial insensitive water channel), localizes to the sarcolemma of fast-twitch muscle fibers. Aquaporins (AQPs) are a large family of integral membrane water transport channel proteins that facilitate the transport of water through the cell membrane. This function is conserved in animals, plants and bacteria. Many isoforms of aquaporin have been identified in mammals, designated AQP0 through AQP10. Aquaporins are widely distributed and it is not uncommon for more than one type of AQP to be present in the same cell. Although most aquaporins are only permeable to water, AQP3, AQP7, AQP9 and one of the two AQP10 transcripts are also permeable to urea and glycerol. AQP2 is the only water channel that is activated by vasopressin to enhance water reabsorption in the kidney collecting duct. Aquaporins are involved in renal water absorption, generation of pulmonary secretions, lacrimation and the secretion and reabsorption of cerebrospinal fluid and aqueous humor. |
| Immunogen | peptide |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHC-P |
| WB:1:100-1,000 IP:1-2 ug per 100-500 ug of total protein (1ml of cell lysate) IF:1:50-1:500 IHC:1:50-1:500 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | AQP 4 antibody AQP-4 antibody AQP4 antibody AQP4_HUMAN antibody Aquaporin type 4 antibody Aquaporin-4 antibody Aquaporin4 antibody HMIWC 2 antibody HMIWC2 antibody Mercurial insensitive water channel antibody Mercurial-insensitive water channel antibody MGC22454 antibody MIWC antibody WCH 4 antibody WCH4 antibody |
|---|---|
| Molecular Weight(MW) | 34 kDa |
| Cellular Localization | Cytoplasm |
| SwissProt ID | P55087 |
|---|
Application
Fig1: Western blot analysis of AQP4 expression in non-transfected (A) and mouse AQP4 transfected (B) 293T whole cell lysates and mouse kidney tissue extract (C).
Application
Fig2： Immunoperoxidase staining of formalin fixed, paraffin-embedded mouse kidney tissue showing membrane localization.| Positive Control | 293T, mouse kidney tissue. |
|---|---|
| Application Notes | WB:1:100-1,000 IP:1-2 ug per 100-500 ug of total protein (1ml of cell lysate) IF:1:50-1:500 IHC:1:50-1:500 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
