Application
Fig1: Western blot analysis of Bim isoform expression in HuT 78 whole cell lysate.Application
Fig2： Immunofluorescence staining of normal mouse lymph node frozen section showing cytoplasmic staining (A). Immunoperoxidase staining of formalin fixed, paraffin-embedded human kidney tissue showing cytoplasmic staining of cells in tubules (B).| Product Name | Bim |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | BCL2 like 11 antibody B2L11_HUMAN antibody BAM antibody Bcl 2 interacting protein Bim antibody Bcl 2 related ovarian death agonist antibody Bcl-2-like protein 11 antibody BCL2 interacting mediator of cell death antibody BCL2 like 11 (apoptosis facilitator) antibody BCL2 like protein 11 antibody Bcl2-interacting mediator of cell death antibody Bcl2-L-11 antibody Bcl2l11 antibody BIM alpha6 antibody BIM antibody BIM beta6 antibody BIM beta7 antibody BimEL antibody BimL antibody BOD antibody |
| Product description | Pro-apototic Bcl-2 family members promote cell death by neutralizing their anti-apoptotic relatives, which otherwise maintain cell viability by regulating caspase activity. Bim belongs to the BH3-only subgroup of Bcl-2 related proteins, and exists in three distinct isoforms, BimS (short), BimL (long) and BimEL (extra long). ERK1/2 phosphorylates BimEL, resulting in rapid degradation of the isoform via the proteasome pathway. At least three sites for ERK1/2 phosphorylation exist on BimEL, whereas ERK1/2 does not effect BimS and BimL, implying a unique role for BimEL in cell survival signaling. |
| Immunogen | peptide |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHC-P |
| WB:1:100-1:1,000 IP:1-2 μg per 100-500 μg of total protein (1 ml of cell lysate) IF:1:50-1:500 IHC:1:50-1:500 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | BCL2 like 11 antibody B2L11_HUMAN antibody BAM antibody Bcl 2 interacting protein Bim antibody Bcl 2 related ovarian death agonist antibody Bcl-2-like protein 11 antibody BCL2 interacting mediator of cell death antibody BCL2 like 11 (apoptosis facilitator) antibody BCL2 like protein 11 antibody Bcl2-interacting mediator of cell death antibody Bcl2-L-11 antibody Bcl2l11 antibody BIM alpha6 antibody BIM antibody BIM beta6 antibody BIM beta7 antibody BimEL antibody BimL antibody BOD antibody |
|---|---|
| Molecular Weight(MW) | BimS: 19kDa, BimL: 21 kDa, BimEL: 24 kDa |
| Cellular Localization | Endomembrane system |
| SwissProt ID | O43521 |
|---|
Application
Fig1: Western blot analysis of Bim isoform expression in HuT 78 whole cell lysate.
Application
Fig2： Immunofluorescence staining of normal mouse lymph node frozen section showing cytoplasmic staining (A). Immunoperoxidase staining of formalin fixed, paraffin-embedded human kidney tissue showing cytoplasmic staining of cells in tubules (B).| Positive Control | HuT 78, human kidney tissue. |
|---|---|
| Application Notes | WB:1:100-1:1,000 IP:1-2 μg per 100-500 μg of total protein (1 ml of cell lysate) IF:1:50-1:500 IHC:1:50-1:500 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
