Application
Fig1: Western blot analysis of GFP fusion protein expression in non-transfected COS cells (A) and COS cells transfected with GFP (B).Application
Fig2： Fluorescence staining of methanol-fixed COS cells transfected with GFP, showing GFP-Protein localization in the cytoplasm.| Product Name | GFP (FL) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | GFP antibody Green fluorescent protein antibody yfp antibody |
| Product description | The green fluorescent protein (GFP) was originally identified as a protein involved in the bioluminescence of the jellyfish Aequorea victoria. GFP cDNA produces a fluorescent product when expressed in prokaryotic cells, without the need for exogenous substrates or cofactors, making GFP a useful tool for monitoring gene expression and protein localization in vivo. Several GFP mutants have been developed, including EGFP, which fluoresce more intensely than the wildtype GFP and have shifted excitation maxima, making them useful for FACS and fluorescence microscopy as well as double-labeling applications. GFP is widely used in expression vectors as a fusion protein tag, allowing expression and monitoring of heterologous proteins fused to GFP. |
| Immunogen | Amino acids 1-238 representing full length GFP of Aequorea victoria origin. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIF |
| WB:1:100-1:1,000 IP:1-2 μg per 100-500 μg of total protein(1 ml of cell lysate) IF:1:50-500 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | GFP antibody Green fluorescent protein antibody yfp antibody |
|---|---|
| Molecular Weight(MW) | 27 kDa |
| Cellular Localization | Cytoplasm |
Application
Fig1: Western blot analysis of GFP fusion protein expression in non-transfected COS cells (A) and COS cells transfected with GFP (B).
Application
Fig2： Fluorescence staining of methanol-fixed COS cells transfected with GFP, showing GFP-Protein localization in the cytoplasm.| Application Notes | WB:1:100-1:1,000 IP:1-2 μg per 100-500 μg of total protein(1 ml of cell lysate) IF:1:50-500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
