Application
Fig1: Western blot analysis of ILK expression in HeLa whole cell lysate.Application
Fig2： Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic staining.| Product Name | ILK |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | 59 kDa serine/threonine protein kinase antibody 59 kDa serine/threonine-protein kinase antibody DKFZp686F1765 antibody Epididymis secretory protein Li 28 antibody HEL S 28 antibody ILK 1 antibody ILK 2 antibody ILK antibody ILK-1 antibody ILK-2 antibody ILK_HUMAN antibody ILK1 antibody ILK2 antibody Integrin linked kinase 2 antibody Integrin linked Kinase antibody Integrin linked protein kinase antibody Integrin-linked protein kinase antibody p59 antibody p59ILK antibody |
| Product description | Integrins are heterodimers composed of non-covalently associated transmembrane α and β subunits. The 16 α and 8 β subunits heterodimerize to produce more than 20 different receptors. Most integrin receptors bind to ligands that are components of the extracellular matrix. Certain integrins can also bind to soluble ligands such as fibrinogen, or to counterreceptors on adjacent cells,such as the intracellular adhesion molecules (ICAMs), leading to aggregation of cells. In addition to mediating cell adhesion and cytoskeletal organization,integrins function as signaling receptors. Signals transduced by integrins play a role in many biological processes, including cell growth, differentiation,migration and apoptosis. ILK (integrin-linked kinase) was identified as a serine/threonine kinase that phosphorylates β1 and β3 integrins. ILK expression has been shown to be reduced in response to fibronectin, a known integrin ligand.Overexpression of ILK was shown to upregulate the fibronectin matrix assembly in epithelial cells, indicating a potential role for ILK in cell growth, cell survival and tumorigenesis. |
| Immunogen | peptide |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIF |
| WB:1:1,000 IP:1-2 μg per 100-500 μg of total protein IF:1:50-500 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | 59 kDa serine/threonine protein kinase antibody 59 kDa serine/threonine-protein kinase antibody DKFZp686F1765 antibody Epididymis secretory protein Li 28 antibody HEL S 28 antibody ILK 1 antibody ILK 2 antibody ILK antibody ILK-1 antibody ILK-2 antibody ILK_HUMAN antibody ILK1 antibody ILK2 antibody Integrin linked kinase 2 antibody Integrin linked Kinase antibody Integrin linked protein kinase antibody Integrin-linked protein kinase antibody p59 antibody p59ILK antibody |
|---|---|
| Molecular Weight(MW) | 59kDa |
| Cellular Localization | Cytoplasm, Cell junction, Cell membrane Cell projection |
| SwissProt ID | Q13418 |
|---|
Application
Fig1: Western blot analysis of ILK expression in HeLa whole cell lysate.
Application
Fig2： Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic staining.| Positive Control | Hela. |
|---|---|
| Application Notes | WB:1:1,000 IP:1-2 μg per 100-500 μg of total protein IF:1:50-500 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
