Application
Fig1: Western blot analysis of IRS-2 expression in HeLa (A) and 3T3-L1 (B) whole cell lysates.Application
Fig2： Immunofluorescence staining of methanol-fixed 3T3-L1 cells showing cytoplasmic staining (A). Immunoperoxidase staining of formalin fixed, paraffin-embedded human skeletal muscle tissue showing cytoplasmic staining of myocytes (B).| Product Name | IRS-2 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Insulin receptor substrate 2 antibody IRS 2 antibody IRS-2 antibody Irs2 antibody IRS2_HUMAN antibody |
| Product description | IRS-2, originally described as 4PS, acts as a signaling intermediate downstream of the Insulin, IGF-1, IL-4, IL-9 and IL-13 receptors. In IRS-2-deficient mice, reduction in total PI 3-kinase activity by 30% and abolition of downstream activation of protein kinase C (PKC) ζ leads to the development of type 2 diabetes. Additionally, reconstitution with retroviral IRS-2 restores IRS-2/PI 3-kinase/PKC ζ signalling as well as glucose uptake. IRS-2 translocates to the nuclei of mouse embryo fibroblasts expressing the Insulin-like growth factor 1 receptor. Various mutations in the IGF-IR can result in an abrogation of or decrease in the translocation of IRS proteins to the nucleoli. IRS-2 is responsible for mitogen-activated protein kinase (MAPK) and protein kinase B (PKB) activation by Insulin and is the major adapter molecule linking the Insulin receptor to this step. |
| Immunogen | Amino acids 926-1130 of IRS-2 of human origin. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIF |
| WB:1:100-1:1,000 IP:1-2 μg per 100-500 μg of total protein (1 ml of cell lysate) IF:1:50-1:500 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | Insulin receptor substrate 2 antibody IRS 2 antibody IRS-2 antibody Irs2 antibody IRS2_HUMAN antibody |
|---|---|
| Molecular Weight(MW) | 165-185kDa |
| Cellular Localization | Cytoplasm |
| SwissProt ID | Q9Y4H2 |
|---|
Application
Fig1: Western blot analysis of IRS-2 expression in HeLa (A) and 3T3-L1 (B) whole cell lysates.
Application
Fig2： Immunofluorescence staining of methanol-fixed 3T3-L1 cells showing cytoplasmic staining (A). Immunoperoxidase staining of formalin fixed, paraffin-embedded human skeletal muscle tissue showing cytoplasmic staining of myocytes (B).| Positive Control | Hela, 3T3-L1, human skeletal muscle tissue. |
|---|---|
| Application Notes | WB:1:100-1:1,000 IP:1-2 μg per 100-500 μg of total protein (1 ml of cell lysate) IF:1:50-1:500 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
