Application
Fig1: Western blot analysis of Ki-67 expression in MCF7 nuclear extract.Application
Fig2： Nuclear Ki67 in mouse mammary gland (60X microscopic magnification in oil). Dilution 1:50 in dilution buffer (0.05% BSA in PBS) Blocking: 0.1% BSA in PBS at room temp.| Product Name | Ki-67 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Antigen identified by monoclonal antibody Ki 67 antibody Antigen identified by monoclonal antibody Ki-67 antibody Antigen KI-67 antibody Antigen KI67 antibody Antigen Ki67 antibody KI67_HUMAN antibody KIA antibody Marker of proliferation Ki-67 antibody MIB 1 antibody MIB antibody MKI67 antibody PPP1R105 antibody Proliferation marker protein Ki-67 antibody Proliferation related Ki 67 antigen antibody Protein phosphatase 1 regulatory subunit 105 antibody RP11-380J17.2 antibody |
| Product description | Ki-67 is a nuclear protein that is expressed in proliferating cells and may be required for maintaining cell proliferation. Ki-67 has been used as a marker for cell proliferation of solid tumors and some hematological malignancies. A correlation has been demonstrated between Ki-67 index and the histopathological grade of neoplasms. Assessment of Ki-67 expression in renal and ureter tumors shows a correlation between tumor proliferation and disease progression, thus making it possible to differentiate high-risk patients. Ki-67 expression may also prove to be important for distinguishing between malignant and benign peripheral nerve sheath tumors. |
| Immunogen | Amino acids 2641-2940 mapping at the C-terminus of Ki-67 of human origin. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHC-P |
| WB:1:100-1:1,000 IP:1-2 μg per 100-500 μg of total protein (1 ml of cell lysate) IF:1:50-1:500 IHC:1:50-1:500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | Antigen identified by monoclonal antibody Ki 67 antibody Antigen identified by monoclonal antibody Ki-67 antibody Antigen KI-67 antibody Antigen KI67 antibody Antigen Ki67 antibody KI67_HUMAN antibody KIA antibody Marker of proliferation Ki-67 antibody MIB 1 antibody MIB antibody MKI67 antibody PPP1R105 antibody Proliferation marker protein Ki-67 antibody Proliferation related Ki 67 antigen antibody Protein phosphatase 1 regulatory subunit 105 antibody RP11-380J17.2 antibody |
|---|---|
| Molecular Weight(MW) | 395/345kDa |
| Cellular Localization | Nucleus, Chromosome |
| SwissProt ID | P46013 |
|---|
Application
Fig1: Western blot analysis of Ki-67 expression in MCF7 nuclear extract.
Application
Fig2： Nuclear Ki67 in mouse mammary gland (60X microscopic magnification in oil). Dilution 1:50 in dilution buffer (0.05% BSA in PBS) Blocking: 0.1% BSA in PBS at room temp.| Positive Control | MCF7, Ki67. |
|---|---|
| Application Notes | WB:1:100-1:1,000 IP:1-2 μg per 100-500 μg of total protein (1 ml of cell lysate) IF:1:50-1:500 IHC:1:50-1:500 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
