Application
Fig1: Western blot analysis ofMAP LC3α/β expression in IMR-32 (A), HeLa (B) and HepG2 (C) whole cell lysates. .| Product Name | LC3A/B |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ATG8F antibody Autophagy related protein LC3 A antibody Autophagy related protein LC3 B antibody Autophagy related ubiquitin like modifier LC3 A antibody Autophagy related ubiquitin like modifier LC3 B antibody Autophagy-related protein LC3 B antibody Autophagy-related ubiquitin-like modifier LC3 B antibody LC3 antibody MAP1 light chain 3 like protein 1 antibody MAP1 light chain 3 like protein 2 antibody MAP1 light chain 3-like protein 2 antibody MAP1A/1B light chain 3 A antibody MAP1A/1B light chain 3 B antibody MAP1A/1BLC3 antibody MAP1A/MAP1B LC3 A antibody MAP1A/MAP1B LC3 B antibody MAP1A/MAP1B light chain 3 B antibody MAP1ALC3 antibody MAP1BLC3 antibody MAP1LC3A antibody Map1lc3b antibody Microtubule associated protein 1 light chain 3 alpha antibody Microtubule associated protein 1 light chain 3 beta antibody Microtubule associated proteins 1A/1B light chain 3 antibody Microtubule associated proteins 1A/1B light chain 3A antibody Microtubule associated proteins 1A/1B light chain 3B antibody Microtubule-associated protein 1 light chain 3 beta antibody Microtubule-associated proteins 1A/1B light chain 3B antibody MLP3B_HUMAN antibody |
| Product description | Microtubule-associated proteins (MAPs) regulate microtubule stability and play critical roles in neuronal development and in maintaining the balance between neuronal plasticity and rigidity. MAP-light chain 3 beta (MAP-LC3β) and MAP-light chain 3 alpha (MAP-LC3α) are subunits that can associate with either MAP-1A or MAP-1B. While MAP-LC3β is essential for autophagy and is associated with autophagosome membranes after processing, MAP LC3α is involved in the formation of autophagosomal vacuoles and is localized to the intracytoplasmic membrane. MAP LC3α is expressed as two alternatively spliced isoforms that are expressed in testis, brain, heart, liver and skeletal muscle, but are absent in thymus and peripheral blood leukocytes. MAP LC3β, which exists in a cytosolic and a membrane-bound form, may also be involved in formation of autophagosomal vacuoles and is expressed primarily in heart, testis, brain and skeletal muscle. |
| Immunogen | Amino acids 58-104 mapping within an internal region of MAP LC3α of human origin. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIF |
| WB:1:100-1:1,000 IP:1-2 μg per 100-500 μg of total protein (1 ml of cell lysate) IF:1:50-1:500 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | ATG8F antibody Autophagy related protein LC3 A antibody Autophagy related protein LC3 B antibody Autophagy related ubiquitin like modifier LC3 A antibody Autophagy related ubiquitin like modifier LC3 B antibody Autophagy-related protein LC3 B antibody Autophagy-related ubiquitin-like modifier LC3 B antibody LC3 antibody MAP1 light chain 3 like protein 1 antibody MAP1 light chain 3 like protein 2 antibody MAP1 light chain 3-like protein 2 antibody MAP1A/1B light chain 3 A antibody MAP1A/1B light chain 3 B antibody MAP1A/1BLC3 antibody MAP1A/MAP1B LC3 A antibody MAP1A/MAP1B LC3 B antibody MAP1A/MAP1B light chain 3 B antibody MAP1ALC3 antibody MAP1BLC3 antibody MAP1LC3A antibody Map1lc3b antibody Microtubule associated protein 1 light chain 3 alpha antibody Microtubule associated protein 1 light chain 3 beta antibody Microtubule associated proteins 1A/1B light chain 3 antibody Microtubule associated proteins 1A/1B light chain 3A antibody Microtubule associated proteins 1A/1B light chain 3B antibody Microtubule-associated protein 1 light chain 3 beta antibody Microtubule-associated proteins 1A/1B light chain 3B antibody MLP3B_HUMAN antibody |
|---|---|
| Molecular Weight(MW) | 15 kDa |
| Cellular Localization | Cytoplasm, Cytoplasmic vesicle Endomembrane system |
| SwissProt ID | Q9H492 |
|---|
Application
Fig1: Western blot analysis ofMAP LC3α/β expression in IMR-32 (A), HeLa (B) and HepG2 (C) whole cell lysates. .| Positive Control | Hela, IMR-32, HepG2e. |
|---|---|
| Application Notes | WB:1:100-1:1,000 IP:1-2 μg per 100-500 μg of total protein (1 ml of cell lysate) IF:1:50-1:500 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
