Application
Fig1: Western blot analysis of paxillin expression in non-transfected 293T (A), human paxillin transfected 293T (B) and HISM (C) whole cell lysates.Application
Fig2： Western blot analysis of paxillin expression in CCD-1064Sk (A) and HISM (B) whole cell lysates.| Product Name | Paxillin |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | FLJ16691 antibody FLJ23042 antibody Paired box protein Pax 1 antibody PAX 1 antibody PAX1 antibody PAXI_HUMAN antibody Paxillin alpha antibody Paxillin antibody PXN antibody PXN protein antibody |
| Product description | Paxillin is a focal adhesion phosphoprotein that is localized to the cytoskeleton. Phosphorylation of paxillin has been shown to occur in response to PDGF treatment, v-src transformation or cross-linking of integrins. FAK (focal adhesion kinase) and PYK2 have been shown to phosphorylate paxillin. FAK phosphorylates paxillin specifically on Tyr-118 in vitro. However, FAK phosphorylation does not seem to be required for the recruitment of paxillin to cell adhesion sites. Paxillin may play a role in signal transduction, regulation of cell morphology and the recruitment of structural and signaling molecules to focal adhesions. It has been shown that the amount of paxillin is reduced in mitotic cells by proteolytic downregulation and that paxillin is alternatively phosphorylated on serine rather than on tyrosine and serine during mitosis. |
| Immunogen | Amino acids 155-268 mapping within an internal region of paxillin of human origin. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIF |
| WB:1:100-1:1,000 IP:1-2 μg per 100-500 μg of total protein (1 ml of cell lysate) IF:1:50-1:500 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | FLJ16691 antibody FLJ23042 antibody Paired box protein Pax 1 antibody PAX 1 antibody PAX1 antibody PAXI_HUMAN antibody Paxillin alpha antibody Paxillin antibody PXN antibody PXN protein antibody |
|---|---|
| Molecular Weight(MW) | 68kDa |
| Cellular Localization | Cytoplasm, Cell junction |
| SwissProt ID | P49023 |
|---|
Application
Fig1: Western blot analysis of paxillin expression in non-transfected 293T (A), human paxillin transfected 293T (B) and HISM (C) whole cell lysates.
Application
Fig2： Western blot analysis of paxillin expression in CCD-1064Sk (A) and HISM (B) whole cell lysates.| Positive Control | 293T, HISM, CCD-1064Sk. |
|---|---|
| Application Notes | WB:1:100-1:1,000 IP:1-2 μg per 100-500 μg of total protein (1 ml of cell lysate) IF:1:50-1:500 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
