Application
Fig1: Western blot analysis of PKAα cat phosphorylation in non-transfected (A) and human PKAα cat transfected (B) 293T whole cell lysates.Application
Fig2： Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear and cytoplasmic localization.| Product Name | Phospho-PKA C (Thr198) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | cAMP dependent protein kinase catalytic subunit alpha antibody cAMP dependent protein kinase catalytic subunit beta antibody cAMP dependent protein kinase catalytic subunit gamma antibody PKA C alpha antibody PKA C beta antibody PKA C gamma antibody PKACA antibody PRKACA antibody PRKACB antibody PRKACG antibody |
| Product description | The second messenger cyclic AMP (cAMP) mediates diverse cellular responses to external signals such as proliferation, ion transport, regulation of metabolism and gene transcription by activation of the cAMP-dependent protein kinase (cAPK or PKA). Activation of PKA occurs when cAMP binds to the two regulatory subunits of the tetrameric PKA holoenzyme resulting in release of active catalytic subunits. Three catalytic (C) subunits have been identified, designated Cα, Cβ and Cγ, that each represent specific gene products. Cα and Cβ are closely related (93% amino acid sequence similarity), whereas Cγ displays 83% and 79% similarity to Cα and Cβ, respectively. Activation of transcription upon elevation of cAMP levels results from translocation of PKA to the nucleus where it phosphorylates the transcription factor cAMP response element binding protein (CREB) on Serine 133, which in turn leads to TFIIB binding to TATA-box-binding protein TBP1, thus linking phospho-CREB to the Pol II transcription initiation complex. The phosphorylation at Threonine 198 is cAMP dependent. |
| Immunogen | A short amino acid sequence containing Thr 198 phosphorylated PKAα cat of human origin. |
| Modification | p-Thr198 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIF |
| WB:1:100-1:1,000 IP:1-2 μg per 100-500 μg of total protein (1 ml of cell lysate) IF:1:50-1:500 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | cAMP dependent protein kinase catalytic subunit alpha antibody cAMP dependent protein kinase catalytic subunit beta antibody cAMP dependent protein kinase catalytic subunit gamma antibody PKA C alpha antibody PKA C beta antibody PKA C gamma antibody PKACA antibody PRKACA antibody PRKACB antibody PRKACG antibody |
|---|---|
| Molecular Weight(MW) | PKAα cat: 40 kDa, PKAβ cat: 53 kDa, PKAγ cat: 39-40 kDa |
| Cellular Localization | Cytoplasm, Nucleus, Membrane, Cell membrane |
| SwissProt ID | P17612 |
|---|
Application
Fig1: Western blot analysis of PKAα cat phosphorylation in non-transfected (A) and human PKAα cat transfected (B) 293T whole cell lysates.
Application
Fig2： Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear and cytoplasmic localization.| Positive Control | Hela, 293T. |
|---|---|
| Application Notes | WB:1:100-1:1,000 IP:1-2 μg per 100-500 μg of total protein (1 ml of cell lysate) IF:1:50-1:500 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
