Application
Fig1: Western blot analysis of S-100 β chain expression in C6 whole cell lysate.Application
Fig2： Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear localization.| Product Name | S100 beta |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | NEF antibody Protein S100 B antibody Protein S100-B antibody S 100 calcium binding protein beta chain antibody S 100 protein beta chain antibody S-100 protein beta chain antibody S-100 protein subunit beta antibody S100 antibody S100 calcium binding protein beta (neural) antibody S100 calcium-binding protein B antibody S100 protein beta chain antibody S100B antibody S100B_HUMAN antibody S100beta antibody |
| Product description | The family of EF-hand type Ca2+-binding proteins includes calbindin (previously designated vitamin D-dependent Ca2+-binding protein), S-100 ? and ∫, calgranulins A (also designated MRP8), B (also designated MRP14) and C (S-100 like proteins), and the parvalbumin family members, including parvalbumin ? and parvalbumin ∫ (also designated oncomodulin). The S-100 protein is involved in the regulation of cellular processes such as cell cycle progression and differentiation. Research also indicates that the S-100 protein may function in the activation of Ca2+ induced Ca2+ release, inhibition of microtubule assembly and inhibition of protein kinase C mediated phosphorylation. Two S-100 subunits, sharing 60% sequence identity, have been described as S-100 ? chain and S-100 ∫ chain. Three S-100 dimeric forms have been characterized, differing in their subunit composition of either two ? chains, two ∫ chains or one ? and one ∫ chain. S-100 localizes to the cytoplasm and nuclei of astrocytes, Schwann's cells, ependymomas and astrogliomas. S-100 is also detected in almost all benign naevi, malignant melanocytic tumours and in Langerhans cells in the skin. Calbindin, S-100 proteins and parvalbumin proteins are each expressed in neural tissues. In addition, S-100 ? and ∫ are present in a variety of other tissues, and calbindin is present in intestine and kidney. |
| Immunogen | Amino acids 37-92 mapping at the C-terminus of S-100 β chain of human origin. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIF |
| WB:1:100-1:1,000 IP:1-2 μg per 100-500 μg of total protein(1 ml of cell lysate) IF:1:50-1:500 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | NEF antibody Protein S100 B antibody Protein S100-B antibody S 100 calcium binding protein beta chain antibody S 100 protein beta chain antibody S-100 protein beta chain antibody S-100 protein subunit beta antibody S100 antibody S100 calcium binding protein beta (neural) antibody S100 calcium-binding protein B antibody S100 protein beta chain antibody S100B antibody S100B_HUMAN antibody S100beta antibody |
|---|---|
| Molecular Weight(MW) | 21kDa |
| Cellular Localization | Cytoplasm, Nucleus |
| SwissProt ID | P04271 |
|---|
Application
Fig1: Western blot analysis of S-100 β chain expression in C6 whole cell lysate.
Application
Fig2： Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear localization.| Positive Control | Hela, C6 |
|---|---|
| Application Notes | WB:1:100-1:1,000 IP:1-2 μg per 100-500 μg of total protein(1 ml of cell lysate) IF:1:50-1:500 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
