Application
Fig1: Western blot analysis of SIRT1 expression in MES-SA/Dx5 whole cell lysate.Application
Fig2： Immunoperoxidase staining of formalin fixed, paraffin-embedded human lung tissue showing nuclear staining of pneumocytes (A). Immunofluorescence staining of methanol-fixed MES- SA/Dx5 cells showing nuclear localization (B).| Product Name | SirT1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | 75SirT1 antibody hSIR2 antibody hSIRT1 antibody HST2, S. cerevisiae, homolog of antibody NAD dependent deacetylase sirtuin 1 antibody NAD dependent protein deacetylase sirtuin 1 antibody OTTHUMP00000198111 antibody OTTHUMP00000198112 antibody Regulatory protein SIR2 homolog 1 antibody SIR1_HUMAN antibody SIR2 antibody SIR2 like 1 antibody SIR2 like protein 1 antibody SIR2, S.cerevisiae, homolog-like 1 antibody SIR2-like protein 1 antibody SIR2ALPHA antibody SIR2L1 antibody Sirt1 antibody SirtT1 75 kDa fragment antibody Sirtuin (silent mating type information regulation 2 homolog) 1 (S. cerevisiae) antibody Sirtuin 1 antibody Sirtuin type 1 antibody |
| Product description | The silent information regulator (SIR2) family of genes are highly-conserved from prokaryotes to eukaryotes and are involved in diverse processes, includ- ing transcriptional regulation, cell cycle progression, DNA-damage repair and aging. In S. cerevisiae, Sir2p deacetylates histones in an NAD-dependent manner , which regulates silencing at the telomeric, rDNA and silent mating- type loci. Sir2p is the founding member of a large family, designated sirtuins which contain a conserved catalytic domain. The human homologs, which include SIRT1-7, are divided into four main branches: SIRT1-3 are class I, SIRT4 is class II, SIRT5 is class III and SIRT6-7 are class IV. SIRT1 has the closest homology to the yeast Sir2p and is widely expressed in fetal and adult tissues, with high expression in heart, brain and skeletal muscle, and low expression in lung and placenta. SIRT1 regulates the p53-dependent DNA damage response pathway by binding to and deacetylating p53, specifi- cally at Lysine 382. |
| Immunogen | Amino acids 448-747 of SIRT1 of human origin. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHC-P |
| WB:1:100-1:1,000 IP:1-2 μg per 100-500 μg of total protein (1 ml of cell lysate) IF:1:50-1:500 IHC:1:50-1:500 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | 75SirT1 antibody
hSIR2 antibody
hSIRT1 antibody
HST2 S. cerevisiae homolog of antibody NAD dependent deacetylase sirtuin 1 antibody NAD dependent protein deacetylase sirtuin 1 antibody OTTHUMP00000198111 antibody OTTHUMP00000198112 antibody Regulatory protein SIR2 homolog 1 antibody SIR1_HUMAN antibody SIR2 antibody SIR2 like 1 antibody SIR2 like protein 1 antibody SIR2 S.cerevisiae homolog-like 1 antibody SIR2-like protein 1 antibody SIR2ALPHA antibody SIR2L1 antibody Sirt1 antibody SirtT1 75 kDa fragment antibody Sirtuin (silent mating type information regulation 2 homolog) 1 (S. cerevisiae) antibody Sirtuin 1 antibody Sirtuin type 1 antibody |
|---|---|
| Molecular Weight(MW) | 120kDa |
| Cellular Localization | Cytoplasm, Nucleus |
| SwissProt ID | Q96EB6 |
|---|
Application
Fig1: Western blot analysis of SIRT1 expression in MES-SA/Dx5 whole cell lysate.
Application
Fig2： Immunoperoxidase staining of formalin fixed, paraffin-embedded human lung tissue showing nuclear staining of pneumocytes (A). Immunofluorescence staining of methanol-fixed MES- SA/Dx5 cells showing nuclear localization (B).| Positive Control | MES-SA/Dx5, human lung tissue |
|---|---|
| Application Notes | WB:1:100-1:1,000 IP:1-2 μg per 100-500 μg of total protein (1 ml of cell lysate) IF:1:50-1:500 IHC:1:50-1:500 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
