Application
Fig1: Western blot analysis of Stat3 expression in non-transfected 293T (A), human Stat3 transfected 293T (B) and K-562 (C) whole cell lysates.Application
Fig2： Immunoperoxidase staining of formalin-fixed, paraffin-embedded human lung tumor showing cytoplasmic and nuclear staining.| Product Name | Stat3 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | 1110034C02Rik antibody Acute Phase Response Factor antibody Acute-phase response factor antibody ADMIO antibody APRF antibody AW109958 antibody DNA binding protein APRF antibody FLJ20882 antibody HIES antibody MGC16063 antibody Signal transducer and activator of transcription 3 (acute phase response factor) antibody Signal transducer and activator of transcription 3 antibody STAT 3 antibody Stat3 antibody STAT3_HUMAN antibody |
| Product description | Membrane receptor signaling by various ligands, including interferons and growth hormones such as EGF , induces activation of Jak kinases which then leads to tyrosine phosphorylation of the various Stat transcription factors. Stat1 and Stat2 are induced by IFN-α and form a heterodimer which is part of the ISGF3 transcription factor complex. Although early reports indicate Stat3 activation by EGF and IL-6, it has been shown that Stat3β appears to be activated by both while Stat3α is activated by EGF, but not by IL-6. Highest expresion of Stat4 is seen in testis and myeloid cells. IL-12 has been identified as an activator of Stat4. Stat5 has been shown to be activated by Prolactin and by IL-3. Stat6 is involved in IL-4 activated signaling pathways. |
| Immunogen | Amino acids 50-240 of Stat3 p92 of human origin. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHC-P |
| WB:1:100-1:1,000 IP:1-2 μg per 100-500 μg of total protein (1 ml of cell lysate) IF:1:50-1:500 IHC:1:50-1:500 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | 1110034C02Rik antibody Acute Phase Response Factor antibody Acute-phase response factor antibody ADMIO antibody APRF antibody AW109958 antibody DNA binding protein APRF antibody FLJ20882 antibody HIES antibody MGC16063 antibody Signal transducer and activator of transcription 3 (acute phase response factor) antibody Signal transducer and activator of transcription 3 antibody STAT 3 antibody Stat3 antibody STAT3_HUMAN antibody |
|---|---|
| Molecular Weight(MW) | 86/91kDa |
| Cellular Localization | Cytoplasm, Nucleus |
| SwissProt ID | P40763 |
|---|
Application
Fig1: Western blot analysis of Stat3 expression in non-transfected 293T (A), human Stat3 transfected 293T (B) and K-562 (C) whole cell lysates.
Application
Fig2： Immunoperoxidase staining of formalin-fixed, paraffin-embedded human lung tumor showing cytoplasmic and nuclear staining.| Positive Control | 293T, K-562 |
|---|---|
| Application Notes | WB:1:100-1:1,000 IP:1-2 μg per 100-500 μg of total protein (1 ml of cell lysate) IF:1:50-1:500 IHC:1:50-1:500 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
