Application
Fig1: Western blot analysis of Stim1 expression in non-transfected 293T (A), human Stim1 transfected 293T (B) and A-10 (C) whole cell lysates.Application
Fig2： Immunoperoxidase staining of formalin fixed, paraffin-embedded human cerebellum tissue showing cytoplasmic and membrane staining of Purkinje cells and cytoplasmic staining of cells in granular layer and cells in molecular layer.| Product Name | STIM1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | D11S4896E antibody GOK antibody OTTHUMP00000164512 antibody OTTHUMP00000229140 antibody OTTHUMP00000230742 antibody SIM antibody STIM 1 antibody STIM1 antibody Stim1 stromal interaction molecule 1 antibody STIM1_HUMAN antibody STIM1L antibody Stromal interaction molecule 1 antibody |
| Product description | Ca2+ influx is essential for a variety of cellular functions, including secretion and transcription. Stromal interaction molecule 1 (Stim1) is a ubiquitously expressed cell surface transmembrane glycoprotein that plays a role in mediating Ca2+ influx following the depletion of intracellular Ca2+ stores. Stim1 functions in the endoplasmic reticulum (ER) where it acts as a Ca2+ sensor via its EF-hand domain, causing large conformational changes. When Ca2+ levels drop, Stim1 translocates from the ER to the plasma membrane, where it activates the calcium release-activated calcium (CRAC) channel subunit, TMEM142A/Orai1. Stim2 is a potent inhibitor of Stim1-mediated, store-operated calcium (SOC) entry. Stim1 is implicated in tumor growth suppression and stromal-haematopoietic cell interactions. |
| Immunogen | Amino acids 441-620 mapping near the C-terminus of Stim1 of human origin. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHC-P |
| WB:1:100-1:1,000 IP:1-2 μg per 100-500 μg of total protein (1 ml of cell lysate) IF:1:50-1:500 IHC:1:50-1:500 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | D11S4896E antibody GOK antibody OTTHUMP00000164512 antibody OTTHUMP00000229140 antibody OTTHUMP00000230742 antibody SIM antibody STIM 1 antibody STIM1 antibody Stim1 stromal interaction molecule 1 antibody STIM1_HUMAN antibody STIM1L antibody Stromal interaction molecule 1 antibody |
|---|---|
| Molecular Weight(MW) | 86kDa |
| Cellular Localization | Cytoplasm, Endoplasmic reticulum membrane Cell membrane |
| SwissProt ID | Q13586 |
|---|
Application
Fig1: Western blot analysis of Stim1 expression in non-transfected 293T (A), human Stim1 transfected 293T (B) and A-10 (C) whole cell lysates.
Application
Fig2： Immunoperoxidase staining of formalin fixed, paraffin-embedded human cerebellum tissue showing cytoplasmic and membrane staining of Purkinje cells and cytoplasmic staining of cells in granular layer and cells in molecular layer.| Positive Control | 293T, A-10, human cerebellum tissue |
|---|---|
| Application Notes | WB:1:100-1:1,000 IP:1-2 μg per 100-500 μg of total protein (1 ml of cell lysate) IF:1:50-1:500 IHC:1:50-1:500 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
