Application
Fig1: Western blot analysis of TTF-1 expression in TT (A), A549 (B) and SHP-77 (C) whole cell lysates.Application
Fig2： Immunoperoxidase staining of formalin fixed, paraffin-embedded human lung tissue showing nuclear staining of pneumocytes (A). Immunofluorescence staining of methanol-fixed TT cells showing nuclear localization (B).| Product Name | TTF1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | AV026640 antibody BCH antibody Benign chorea antibody BHC antibody Homeobox protein NK 2 homolog A antibody Homeobox protein NK-2 homolog A antibody Homeobox protein Nkx 2.1 antibody Homeobox protein Nkx-2.1 antibody Homeobox protein Nkx2.1 antibody NK 2 antibody NK 2 homolog A antibody NK2 antibody NK2 homeobox 1 antibody NK2, drosophila, homolog of, A antibody NK2.1, mouse, homolog of antibody Nkx 2 1 antibody NKX 2.1 antibody NKX 2A antibody NKX2 1 antibody Nkx2-1 antibody NKX2.1 antibody NKX21_HUMAN antibody NKX2A antibody T EBP antibody T/EBP antibody TEBP antibody Thyroid nuclear factor 1 antibody Thyroid nuclear factor antibody Thyroid specific enhancer binding protein antibody Thyroid transcription factor 1 antibody Tin man antibody Tinman antibody TITF 1 antibody TITF1 antibody TTF 1 antibody TTF-1 antibody TTF1 antibody |
| Product description | TTF-1 (thyroid transcription factor-1, BCH, BHC, NK-2, Nkx2.1, Nkx2A, TEBP, TTF1) is a member of the Nkx2 family of homeodomain-containing transcription factors and regulates the transcriptional activity of thyroid-specific genes. TTF-1 influences organogenisis and the maintenance of the differentiated phenotypes of various tissues including thyroid, lung and brain. TTF-1, which is present in the epithelium of the lung, regulates transcription of the surfactant proteins (SP) A, B and C and is essential for lung morphogenesis. In the thyroid, TTF-1 elevates the expression of thyroid specific markers, thyroglobulin, thyroperoxidase and thyrotropin receptors. TTF-1 interacts with SRC-1 and CBP in vitro. |
| Immunogen | Amino acids 1-190 mapping at the N-terminus of TTF-1 of human origin. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHC-P |
| WB:1:100-1:1,000 IP:1-2 μg per 100-500 μg of total protein (1 ml of cell lysate) IF:1:50-1:500 IHC:1:50-1:500 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | AV026640 antibody
BCH antibody
Benign chorea antibody
BHC antibody
Homeobox protein NK 2 homolog A antibody
Homeobox protein NK-2 homolog A antibody
Homeobox protein Nkx 2.1 antibody
Homeobox protein Nkx-2.1 antibody
Homeobox protein Nkx2.1 antibody
NK 2 antibody
NK 2 homolog A antibody
NK2 antibody
NK2 homeobox 1 antibody
NK2 drosophila homolog of A antibody NK2.1 mouse homolog of antibody Nkx 2 1 antibody NKX 2.1 antibody NKX 2A antibody NKX2 1 antibody Nkx2-1 antibody NKX2.1 antibody NKX21_HUMAN antibody NKX2A antibody T EBP antibody T/EBP antibody TEBP antibody Thyroid nuclear factor 1 antibody Thyroid nuclear factor antibody Thyroid specific enhancer binding protein antibody Thyroid transcription factor 1 antibody Tin man antibody Tinman antibody TITF 1 antibody TITF1 antibody TTF 1 antibody TTF-1 antibody TTF1 antibody |
|---|---|
| Molecular Weight(MW) | 348 kDa |
| Cellular Localization | Nucleus |
| SwissProt ID | P43699 |
|---|
Application
Fig1: Western blot analysis of TTF-1 expression in TT (A), A549 (B) and SHP-77 (C) whole cell lysates.
Application
Fig2： Immunoperoxidase staining of formalin fixed, paraffin-embedded human lung tissue showing nuclear staining of pneumocytes (A). Immunofluorescence staining of methanol-fixed TT cells showing nuclear localization (B).| Positive Control | TT, A549, SHP-77, human lung tissue. |
|---|---|
| Application Notes | WB:1:100-1:1,000 IP:1-2 μg per 100-500 μg of total protein (1 ml of cell lysate) IF:1:50-1:500 IHC:1:50-1:500 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
