Application
Fig1: Western blot analysis of UBC9 expression in Jurkat whole cell lysate.Application
Fig2： Western blot analysis of UBC9 expression in HeLa whole cell lysate.| Product Name | UBC9 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | C358B7.1 antibody p18 antibody SUMO 1 protein ligase antibody SUMO conjugating enzyme UBC9 antibody SUMO-conjugating enzyme UBC9 antibody SUMO-protein ligase antibody SUMO1 protein ligase antibody UBC9 antibody UBC9_HUMAN antibody UBCE9 antibody Ube2i antibody Ubiquitin carrier protein 9 antibody Ubiquitin carrier protein antibody Ubiquitin carrier protein I antibody Ubiquitin conjugating enzyme 9 antibody Ubiquitin conjugating enzyme E2I (homologous to yeast UBC9) antibody Ubiquitin conjugating enzyme E2I (UBC9 homolog, yeast) antibody Ubiquitin conjugating enzyme UbcE2A antibody Ubiquitin like protein SUMO 1 conjugating enzyme antibody Ubiquitin protein ligase E2I antibody Ubiquitin-conjugating enzyme E2 I antibody Ubiquitin-protein ligase I antibody |
| Product description | UBC9 is a component of the ubiquitin-mediated proteolytic pathway, which targets proteins for degradation by the 26S proteasome, mediates endocytosis and directs protein subcellular localization. Ub and Ub-like molecules are systematically transferred from E2 conjugating enzymes to the targeted substrate by way of an E3 ubiquitin ligase. UBC9 functions as an E2 ubiquitin conjugating enzyme that preferentially associates with the ubiquitin homolog designated SUMO-1 or sentrin, a component of the sentrinization complex. Characteristic of the E2 family members, UBC9 contains a conserved cysteine residue that is required for the thio ester formation between Ub-like proteins and the E2 member, and it shares a conserved UBC domain. Substrates for UBC9 include transcription factors E12 and E47 and mitotic regulators RanBP2 and RanGAP1, which indicates that UBC9 may regulate various cellular processes including cell cycle progression and differentiation. |
| Immunogen | Amino acids 1-81 of UBC9 of human origin. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHC-P |
| WB:1:100-1:1,000 IP:1-2 μg per 100-500 μg of total protein (1 ml of cell lysate) IF:1:50-1:500 IHC:1:50-1:500 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | C358B7.1 antibody
p18 antibody
SUMO 1 protein ligase antibody
SUMO conjugating enzyme UBC9 antibody
SUMO-conjugating enzyme UBC9 antibody
SUMO-protein ligase antibody
SUMO1 protein ligase antibody
UBC9 antibody
UBC9_HUMAN antibody
UBCE9 antibody
Ube2i antibody
Ubiquitin carrier protein 9 antibody
Ubiquitin carrier protein antibody
Ubiquitin carrier protein I antibody
Ubiquitin conjugating enzyme 9 antibody
Ubiquitin conjugating enzyme E2I (homologous to yeast UBC9) antibody
Ubiquitin conjugating enzyme E2I (UBC9 homolog yeast) antibody Ubiquitin conjugating enzyme UbcE2A antibody Ubiquitin like protein SUMO 1 conjugating enzyme antibody Ubiquitin protein ligase E2I antibody Ubiquitin-conjugating enzyme E2 I antibody Ubiquitin-protein ligase I antibody |
|---|---|
| Molecular Weight(MW) | 18 kDa |
| Cellular Localization | Cytoplasm, Nucleus |
| SwissProt ID | P63279 |
|---|
Application
Fig1: Western blot analysis of UBC9 expression in Jurkat whole cell lysate.
Application
Fig2： Western blot analysis of UBC9 expression in HeLa whole cell lysate.| Positive Control | Hela, Jurkat. |
|---|---|
| Application Notes | WB:1:100-1:1,000 IP:1-2 μg per 100-500 μg of total protein (1 ml of cell lysate) IF:1:50-1:500 IHC:1:50-1:500 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
