Application
Fig1: Western blot analysis of MIF expression in non-transfected 293T (A), human MIF transfected 293T (B) and Y79 (C) whole cell lysates.Application
Fig2： Immunoperoxidase staining of formalin fixed, paraffin-embedded mouse kidney tissue showing cytoplasmic and extracellular localization (A). Immunoperoxidase staining of formalin fixed, paraffinembedded human prostate tissue showing cytoplasmic and membrane staining of glandular cells(B).| Product Name | MIF |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | GIF antibody GLIF antibody Glycosylation inhibiting factor antibody Glycosylation-inhibiting factor antibody L-dopachrome isomerase antibody L-dopachrome tautomerase antibody Macrophage migration inhibitory factor (glycosylation-inhibiting factor) antibody Macrophage migration inhibitory factor antibody MIF antibody MIF protein antibody MIF_HUMAN antibody MMIF antibody Phenylpyruvate tautomerase antibody |
| Product description | Macrophage migration inhibitory factor, known as MIF or glycosylation-inhibiting factor, is a secreted, homotrimeric, pro-inflammatory cytokine that modulates macrophage and T cell function and is an important regulator of host response to infection. MIF is expressed at sites of inflammation, which suggests that it plays a role in regulating macrophage function in host defense. MIF is produced by the pituitary gland and is found in monocytes, macrophages, differentiating immunological cells in the eye lens and brain, and fibroblasts. Elevated levels of MIF protein are detected in the plasma of patients with severe sepsis or septic shock, a condition where MIF influences endotoxic shock by enhancing the production of other inflammatory cytokines including tumor necrosis factor α (TNFα), interleukin-1 (IL-1) and interferon-γ (IFN-γ). MIF promotes the systemic inflammatory response by counter-regulating glucocorticoid-mediated inhibition of immune-cell activation and proinflammatory cytokine production. MIF may mediate tissue destruction through the induction of proteinases. |
| Immunogen | peptide |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHC-P |
| WB:1:100-1:1,000 IP:1-2 μg per 100-500 μg of total protein (1 ml of cell lysate) IF:1:50-1:500 IHC:1:50-1:500 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | GIF antibody GLIF antibody Glycosylation inhibiting factor antibody Glycosylation-inhibiting factor antibody L-dopachrome isomerase antibody L-dopachrome tautomerase antibody Macrophage migration inhibitory factor (glycosylation-inhibiting factor) antibody Macrophage migration inhibitory factor antibody MIF antibody MIF protein antibody MIF_HUMAN antibody MMIF antibody Phenylpyruvate tautomerase antibody |
|---|---|
| Molecular Weight(MW) | 12.5kDa |
| Cellular Localization | Cytoplasm, Secreted |
| SwissProt ID | P14174 |
|---|
Application
Fig1: Western blot analysis of MIF expression in non-transfected 293T (A), human MIF transfected 293T (B) and Y79 (C) whole cell lysates.
Application
Fig2： Immunoperoxidase staining of formalin fixed, paraffin-embedded mouse kidney tissue showing cytoplasmic and extracellular localization (A). Immunoperoxidase staining of formalin fixed, paraffinembedded human prostate tissue showing cytoplasmic and membrane staining of glandular cells(B).| Positive Control | Y79, mouse kidney tissue, human prostate tissue. |
|---|---|
| Application Notes | WB:1:100-1:1,000 IP:1-2 μg per 100-500 μg of total protein (1 ml of cell lysate) IF:1:50-1:500 IHC:1:50-1:500 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
