WB
Western blot analysis using PSAP mouse mAb against HEK293 (1), C6 (2), and HT1080 (3) cell lysate.IHC
Immunohistochemical analysis of paraffin-embedded human prostate tissues using PSAP mouse mAb with DAB staining.ICC/IF
Immunofluorescence analysis of Hela cells using PSAP mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.FC
Flow cytometric analysis of Hela cells using PSAP mouse mAb (green) and negative control (red).| Product Name | Mouse Monoclonal Antibody to PSAP |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Purified recombinant fragment of human PSAP (AA: 17-216) expressed in E. Coli. |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | FCICC/IFIHCWB |
| WB:1:500-1:2000 IHC:1:200-1:1000 ICC/IF:1:200-1:1000 ICC/IF:1:200-1:400 |
|
| Species Reactivity | HumanRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PSAP |
|---|---|
| Gene Synonyms | GLBA SAP1 SAP2 PSAPD PARK24 |
| Gene Full Name | prosaposin |
| Gene Summary | This gene encodes a highly conserved preproprotein that is proteolytically processed to generate four main cleavage products including saposins A, B, C, and D. Each domain of the precursor protein is approximately 80 amino acid residues long with nearly identical placement of cysteine residues and glycosylation sites. Saposins A-D localize primarily to the lysosomal compartment where they facilitate the catabolism of glycosphingolipids with short oligosaccharide groups. The precursor protein exists both as a secretory protein and as an integral membrane protein and has neurotrophic activities. Mutations in this gene have been associated with Gaucher disease and metachromatic leukodystrophy. Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed. [provided by RefSeq, Feb 2016] |
| Molecular Weight(MW) | 58.1kDa |
| Cellular Localization | Lysosome,Secreted. |
| Entrez Gene | 5660 |
|---|
WB
Western blot analysis using PSAP mouse mAb against HEK293 (1), C6 (2), and HT1080 (3) cell lysate.
IHC
Immunohistochemical analysis of paraffin-embedded human prostate tissues using PSAP mouse mAb with DAB staining.
ICC/IF
Immunofluorescence analysis of Hela cells using PSAP mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
FC
Flow cytometric analysis of Hela cells using PSAP mouse mAb (green) and negative control (red).| Application Notes | WB:1:500-1:2000 IHC:1:200-1:1000 ICC/IF:1:200-1:1000 ICC/IF:1:200-1:400 |
|---|
| Form | Purified antibody in PBS with 0.05% sodium azide |
|---|---|
| Storage Instructions | 4℃; -20℃ for long term storage |
