WB
Figure 1: Western blot analysis using AKT2 mouse mAb against A431 (1), Jurkat (2), HEK293 (3), A549 (4), MCF-7 (5) and PC-12 (6) cell lysate.IHC
Figure 2: Immunohistochemical analysis of paraffin-embedded human Thymus tissues using anti-AKT2 mAbICC/IF
Figure 3: Immunofluorescence analysis of PANC-1 (left) and Hela (right) cells using AKT2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.| Product Name | Mouse Monoclonal Antibody to AKT2 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Purified recombinant fragment of human AKT2 expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2b |
| Host Species | Mouse |
| Tested Applications | ICC/IFIHCWB |
| WB:1:250-1:1000 IHC:1:20-1:100 ICC/IF:1:100-1:200 |
|
| Species Reactivity | HumanMonkeyRat |
| Purification | Affinity purified |
| Gene Symbol | AKT2 |
|---|---|
| Gene Synonyms | PKBB PRKBB HIHGHH PKBBETA RAC-BETA |
| Gene Full Name | AKT serine/threonine kinase 2 |
| Gene Summary | This gene is a putative oncogene encoding a protein belonging to a subfamily of serine/threonine kinases containing SH2-like (Src homology 2-like) domains, which is involved in signaling pathways. The gene serves as an oncogene in the tumorigenesis of cancer cells For example, its overexpression contributes to the malignant phenotype of a subset of human ductal pancreatic cancers. The encoded protein is a general protein kinase capable of phophorylating several known proteins, and has also been implicated in insulin signaling. [provided by RefSeq, Nov 2019] |
| Molecular Weight(MW) | 60 kDa |
| Cellular Localization | Cell membrane,Cytoplasm,Endosome,Membrane,Nucleus |
WB
Figure 1: Western blot analysis using AKT2 mouse mAb against A431 (1), Jurkat (2), HEK293 (3), A549 (4), MCF-7 (5) and PC-12 (6) cell lysate.
IHC
Figure 2: Immunohistochemical analysis of paraffin-embedded human Thymus tissues using anti-AKT2 mAb
ICC/IF
Figure 3: Immunofluorescence analysis of PANC-1 (left) and Hela (right) cells using AKT2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.| Application Notes | WB:1:250-1:1000 IHC:1:20-1:100 ICC/IF:1:100-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 4℃; -20℃ for long term storage |
| Storage Buffer | Purified antibody in PBS with 0.05% sodium azide. |
