WB
Western Blot analysis using FLAG-tag Polyclonal Antibody against HEK293 cells transfected with vector overexpressing FLAG tag (1) and untransfected (2). Secondary antibody was diluted at 1:20000| Product Name | DDDDK-Tag(binds to flag sequnence) rabbit pAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | FLAG-tag Polyclonal Antibody detects FLAG-tagged recombinant proteins or FLAG-tagged proteins overexpressed in cells. |
| Immunogen | DDDDK synthetic peptide conjugated to KLH. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICC/IFIPWB |
| WB: 1:1000-3000 ELISA: 1:5000-20000 IF:1:50-200 |
|
| Species Reactivity | A wide range of other species |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | FLAG-tag |
|---|---|
| Gene Synonyms | Flag tag Flag-tag DDDDK TAG DDDDK-TAG DYKDDDDK tag DYKDDDDK-tag |
| Target Description | The DYKDDDDK (FLAG) peptide has been used extensively as a general tag in expression vectors. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion. N-terminal FLAG vectors provide an Ek cleavage site for removal of the fusion tag. The FLAG peptide is likely to be located on the surface of a fusion protein because of its hydrophilic nature. As a result, the FLAG peptide is more likely to be accessible to antibodies. A FLAG-tag can be used in many different assays that require recognition by an antibody, such as western blotting, immunocytochemistry, immunoprecipitation, flow cytometry, protein purification, and in the study of protein-protein interactions, cell ultrastructure, and protein localization and so on. |
WB
Western Blot analysis using FLAG-tag Polyclonal Antibody against HEK293 cells transfected with vector overexpressing FLAG tag (1) and untransfected (2). Secondary antibody was diluted at 1:20000| Application Notes | WB: 1:1000-3000 ELISA: 1:5000-20000 IF:1:50-200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Storage Buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
