WB
Western Blot analysis of 293F and CHO cells transfected or non-tansfected DDDK-tag expression vector by primary antibody at 1:3000 dilution.WB
Western Blot analysis of flag-tag protein using primary antibody at various dilution. Secondary antibody was diluted at 1:10000| Product Name | DDDDK-Tag(binds to flag sequnence) Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | This recombinant antibody can highly specifically recognize the Flag tags of C-terminal and N-terminal of recombinant protein. Variable region gene of this antibody comes from the mouse gene library immunized with DDDK peptide. Constant region of this antibody is mouse IgG1 and mouse kappa. |
| Immunogen | Synthetic Peptide of Flag-Tag |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICC/IFWB |
| WB: 1:1000-5000 ELISA: 1:20000-50000 IF:1:100-300 |
|
| Species Reactivity | A wide range of other species |
| Purification | Affinity purified |
| Gene Symbol | Flag-tag |
|---|---|
| Gene Synonyms | Flag tag Flag-tag DDDDK TAG DDDDK-TAG DYKDDDDK tag DYKDDDDK-tag |
| Target | The antibody detects C-terminal, internal, and N-terminal Flag-tag fusion proteins. |
| Target Description | The DYKDDDDK peptide (Flag-tag) is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, and then used to separate recombinant, over expressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits. |
WB
Western Blot analysis of 293F and CHO cells transfected or non-tansfected DDDK-tag expression vector by primary antibody at 1:3000 dilution.
WB
Western Blot analysis of flag-tag protein using primary antibody at various dilution. Secondary antibody was diluted at 1:10000| Application Notes | WB: 1:1000-5000 ELISA: 1:20000-50000 IF:1:100-300 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Storage Buffer | PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol. |
