WB
Western blot analysis of various lysates using ATP23 Rabbit pAb at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L)at 1:10000 dilution. Lysates / proteins: 25ug per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.IHC
Immunohistochemistry analysis of ATP23 in paraffin-embedded rat kidney tissue using ATP23 Rabbit pAb at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.IHC
Immunohistochemistry analysis of ATP23 in paraffin-embedded rat heart tissue using ATP23 Rabbit pAb at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.ICC/IF
Immunofluorescence analysis of MCF7 cells using ATP23 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L)at 1:500 dilution. Blue: DAPI for nuclear staining.| Product Name | ATP23 Rabbit pAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 1-246 of human ATP23 (NP_150592.1). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICC/IFIHCWB |
| WB:1:1000-1:3000 IHC:1:50-1:200 ICC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Purification | Affinity purified |
| Gene Symbol | ATP23 |
|---|---|
| Gene Synonyms | KUB3 XRCC6BP1 |
| Gene Full Name | ATP23 metallopeptidase and ATP synthase assembly factor homolog |
| Gene Summary | The protein encoded by this gene is amplified in glioblastomas and interacts with the DNA binding subunit of DNA-dependent protein kinase. This kinase is involved in double-strand break repair (DSB), and higher expression of the encoded protein increases the efficiency of DSB. In addition, comparison to orthologous proteins strongly suggests that this protein is a metalloprotease important in the biosynthesis of mitochondrial ATPase. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2016] |
| Molecular Weight(MW) | 28KD |
WB
Western blot analysis of various lysates using ATP23 Rabbit pAb at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L)at 1:10000 dilution. Lysates / proteins: 25ug per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
IHC
Immunohistochemistry analysis of ATP23 in paraffin-embedded rat kidney tissue using ATP23 Rabbit pAb at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
IHC
Immunohistochemistry analysis of ATP23 in paraffin-embedded rat heart tissue using ATP23 Rabbit pAb at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ICC/IF
Immunofluorescence analysis of MCF7 cells using ATP23 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L)at 1:500 dilution. Blue: DAPI for nuclear staining.| Application Notes | WB:1:1000-1:3000 IHC:1:50-1:200 ICC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS with 0.01% thimerosal,50% glycerol,pH7.3. |
