WB
Western blot analysis of lysates from wild type (WT) and 293T cells transfected with CGRP using CGRP Rabbit pAb at 1:2000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.IHC
Immunohistochemistry analysis of paraffinembedded Human thyroid medullary carcinoma tissue using CGRP Rabbit pAb at a dilution of 1:6000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.ICC/IF
Immunofluorescence analysis of L929 cells using CGRP Rabbit pAb at dilution of 1:100. Blue: DAPI for nuclear staining.| Product Name | CGRP Rabbit pAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | A synthetic peptide corresponding to a sequence within amino acids 49-128 of human CGRP (NP_001029125.1). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:1000-1:3000 IHC:1:50-1:200 ICC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Purification | Affinity purified |
| Gene Symbol | CALCA |
|---|---|
| Gene Synonyms | CT KC PCT CGRP CALC1 CGRP1 CGRP-I CGRP-alpha |
| Gene Full Name | calcitonin related polypeptide alpha |
| Gene Summary | This gene encodes the peptide hormones calcitonin, calcitonin gene-related peptide and katacalcin by tissue-specific alternative RNA splicing of the gene transcripts and cleavage of inactive precursor proteins. Calcitonin is involved in calcium regulation and acts to regulate phosphorus metabolism. Calcitonin gene-related peptide functions as a vasodilator and as an antimicrobial peptide while katacalcin is a calcium-lowering peptide. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Aug 2014] |
| Molecular Weight(MW) | 14kDa |
WB
Western blot analysis of lysates from wild type (WT) and 293T cells transfected with CGRP using CGRP Rabbit pAb at 1:2000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
IHC
Immunohistochemistry analysis of paraffinembedded Human thyroid medullary carcinoma tissue using CGRP Rabbit pAb at a dilution of 1:6000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.
ICC/IF
Immunofluorescence analysis of L929 cells using CGRP Rabbit pAb at dilution of 1:100. Blue: DAPI for nuclear staining.| Application Notes | WB:1:1000-1:3000 IHC:1:50-1:200 ICC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS with 0.01% thimerosal,50% glycerol,pH7.3. |
