WB
Western blot analysis of lysates from HepG2 cells, using ATP6 Rabbit pAb at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 20s.ICC/IF
Immunofluorescence analysis of U2OS cells using ATP6 Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3 Goat AntiRabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.| Product Name | ATP6 Rabbit pAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | A synthetic peptide corresponding to a sequence within amino acids 127-226 of mouse ATP6 (NP_904333.1). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:500-1:2000 IHC:1:100-1:200 ICC:1:50-1:200 |
|
| Species Reactivity | HumanMouse |
| Purification | Affinity purified |
| Gene Symbol | mt-Atp6 |
|---|---|
| Gene Synonyms | ATP6 |
| Gene Full Name | ATP synthase 6, mitochondrial |
| Gene Summary | Predicted to contribute to proton-transporting ATP synthase activity, rotational mechanism. Predicted to be involved in mitochondrial ATP synthesis coupled proton transport. Located in mitochondrion. Is expressed in several structures, including alimentary system; brain; heart; integumental system; and sensory organ. Human ortholog(s) of this gene implicated in Leber hereditary optic neuropathy; NARP syndrome; Parkinson's disease; multiple sclerosis; and systemic lupus erythematosus. Orthologous to human MT-ATP6 (mitochondrially encoded ATP synthase membrane subunit 6). [provided by Alliance of Genome Resources, Apr 2022] |
| Molecular Weight(MW) | 25kDa(Observed MW 20kDa) |
| Cellular Localization | Mitochondrion inner membrane,Multi-pass membrane protein |
WB
Western blot analysis of lysates from HepG2 cells, using ATP6 Rabbit pAb at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 20s.
ICC/IF
Immunofluorescence analysis of U2OS cells using ATP6 Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3 Goat AntiRabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.| Application Notes | WB:1:500-1:2000 IHC:1:100-1:200 ICC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3. |
