| Product Name | GAPDH Monoclonal Antibody(2B8), AbFluor 488 Conjugated |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | ICC/IFIHCWB |
| IHC:1:200 IF:1:200 |
|
| Species Reactivity | DogHumanMousePigRabbitRatSheep |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | GAPDH |
|---|---|
| Gene Synonyms | G3PD GAPD HEL-S-162eP |
| Gene Full Name | glyceraldehyde-3-phosphate dehydrogenase |
| Gene Summary | This gene encodes a member of the glyceraldehyde-3-phosphate dehydrogenase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The encoded protein has additionally been identified to have uracil DNA glycosylase activity in the nucleus. Also, this protein contains a peptide that has antimicrobial activity against E. coli, P. aeruginosa, and C. albicans. Studies of a similar protein in mouse have assigned a variety of additional functions including nitrosylation of nuclear proteins, the regulation of mRNA stability, and acting as a transferrin receptor on the cell surface of macrophage. Many pseudogenes similar to this locus are present in the human genome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2014] |
| Cellular Localization | Cytoplasm, cytosol . Nucleus . Cytoplasm, perinuclear region . Membrane . Cytoplasm, cytoskeleton . Translocates to the nucleus following S-nitrosylation and interaction with SIAH1, which contains a nuclear localization signal (By similarity). Postnuclear and Perinuclear regions (PubMed:12829261). |
| Application Notes | IHC:1:200 IF:1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Stable for one year at -15°C to -25°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing. |
| Storage Buffer | In PBS pH 7.4, containing 0.02% sodium azide as preservative and 50% Glycerol |
