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Caspase-8 Recombinant Rabbit Monoclonal Antibody
Catalog Number:OM642823
OM642823
Discount Price
OM642823-100ul
1~2 Week
100ul
OM642823-50ul
50ul
OM642823-20ul
20ul
1~2 Week
Product Profile
Product Name Caspase-8 Recombinant Rabbit Monoclonal Antibody
Antibody Type Primary Antibodies
Immunogen Synthetic peptide within Human Caspase-8 aa 200-249 / 479.
Key Feature
Clonality monoclonal
Isotype IgG
Host Species Rabbit
Tested Applications IHCWB
WB:1:1000-1:2000
IHC:1:50-1:200
ICC/IF:1:50-1:200
IF-F:1:50-1:200
Species Reactivity Human
Concentration 1mg/ml
Purification Protein A
Target Information
Gene Symbol CASP8
Gene Synonyms CAP4
MACH
MCH5
FLICE
ALPS2B
Casp-8
Gene Full Name caspase 8
Gene Summary This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes composed of a prodomain, a large protease subunit, and a small protease subunit. Activation of caspases requires proteolytic processing at conserved internal aspartic residues to generate a heterodimeric enzyme consisting of the large and small subunits. This protein is involved in the programmed cell death induced by Fas and various apoptotic stimuli. The N-terminal FADD-like death effector domain of this protein suggests that it may interact with Fas-interacting protein FADD. This protein was detected in the insoluble fraction of the affected brain region from Huntington disease patients but not in those from normal controls, which implicated the role in neurodegenerative diseases. Many alternatively spliced transcript variants encoding different isoforms have been described, although not all variants have had their full-length sequences determined. [provided by RefSeq, Jul 2008]
Molecular Weight(MW) 55kDa
Cellular Localization Cytoplasm, Nucleus.
Application

WB

Western blot analysis of Caspase-8 on different lysates with Rabbit anti-Caspase-8 antibody at 1/1,000 dilution. Lane 1: Jurkat cell lysate, Lane 2: HeLa cell lysate, Lane 3: THP-1 cell lysate, Lane 4: HepG2 cell lysate, Lane 5: 293T cell lysate, Lane 6: SH-SY5Y cell lysate (negative), Lysates/proteins at 20 µg/Lane. Exposure time: 40 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

IHC

Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Caspase-8 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

IHC

Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Caspase-8 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Application Notes WB:1:1000-1:2000
IHC:1:50-1:200
ICC/IF:1:50-1:200
IF-F:1:50-1:200
Additional Information
Form Liquid
Storage Instructions Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage Buffer 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
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