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Mouse Monoclonal Antibody to Cleaved-Caspase 8
Catalog Number:OM644123
OM644123
Discount Price
OM644123-100ul
100ul
OM644123-50ul
50ul
Product Profile
Product Name Mouse Monoclonal Antibody to Cleaved-Caspase 8
Antibody Type Primary Antibodies
Immunogen Recombinant Protein of Caspase-8.
Key Feature
Clonality monoclonal
Isotype IgG1
Host Species Mouse
Tested Applications ICC/IFIHCWB
WB:1:500-1:1000
IHC:1:50-1:100
ICC/IF:1:50-1:200
Species Reactivity HumanMouseRat
Concentration 1mg/ml
Purification Affinity purified
Target Information
Gene Symbol CASP8
Gene Synonyms CAP4
MACH
MCH5
FLICE
ALPS2B
Casp-8
Gene Full Name caspase 8
Gene Summary This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes composed of a prodomain, a large protease subunit, and a small protease subunit. Activation of caspases requires proteolytic processing at conserved internal aspartic residues to generate a heterodimeric enzyme consisting of the large and small subunits. This protein is involved in the programmed cell death induced by Fas and various apoptotic stimuli. The N-terminal FADD-like death effector domain of this protein suggests that it may interact with Fas-interacting protein FADD. This protein was detected in the insoluble fraction of the affected brain region from Huntington disease patients but not in those from normal controls, which implicated the role in neurodegenerative diseases. Many alternatively spliced transcript variants encoding different isoforms have been described, although not all variants have had their full-length sequences determined. [provided by RefSeq, Jul 2008]
Molecular Weight(MW) 55kDa(Observed MW: 43kDa)
Cellular Localization Cytoplasm,Nucleus.
Application

WB

Western blot analysis of Caspase 8 in THP-1, rat Brain, Hela lysates using Caspase8 antibody.

IHC

Immunohistochemistry analysis of paraffin-embedded mouse Spleen Tissue using Caspase8 antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

ICC/IF

Immunocytochemistry analysis of Caspase8 (green) in Hela using Caspase8 antibody,and DAPI(blue).
Application Notes WB:1:500-1:1000
IHC:1:50-1:100
ICC/IF:1:50-1:200
Additional Information
Form Liquid
Storage Instructions Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Storage Buffer Purified antibody in PBS with 0.05% sodium azide.
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