WB
Western blot analysis of extracts from various samples, using pan-AKT1/2/3 Antibody. Lane 1: Rat muscle, blocked with antigen-specific peptides, Lane 2: Rat muscle, Lane 3: Mouse brain, Lane 4: A549 cells(heat-shock treatment), Lane 5: RAW264.7 cells(H2O2 treatment).IHC
pan-AKT1/2/3 Antibody at 1/100 staining Human normal tissues adjacent to mammary cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.ICC/IF
pan-AKT1/2/3 Antibody staining A549 cells by IF/ICC.The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab and mouse anti-beta tubulin Ab for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody. The nuclear counter stain is DAPI(blue).| Product Name | pan-AKT1/2/3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | A synthesized peptide derived from human AKT1(Accession P31749), corresponding to amino acid residues P424-Y474. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:500-1:2000 IHC:1:50-1:200 IF/ICC:1:100-1:500 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | AKT1/2/3 |
|---|---|
| Target | pan-AKT1/2/3 Antibody detects endogenous levels of total pan-AKT1/2/3. |
| Molecular Weight(MW) | 55kDa; 56kD(Calculated) |
| Tissue Specificity | Expressed in prostate cancer and levels increase from the normal to the malignant state (at protein level). Expressed in all human cell types so far analyzed. The Tyr-176 phosphorylated form shows a significant increase in expression in breast cancers during the progressive stages i.e. normal to hyperplasia (ADH), ductal carcinoma in situ (DCIS), invasive ductal carcinoma (IDC) and lymph node metastatic (LNMM) stages. |
| Cellular Localization | Cytoplasm. Nucleus. Cell membrane. |
WB
Western blot analysis of extracts from various samples, using pan-AKT1/2/3 Antibody. Lane 1: Rat muscle, blocked with antigen-specific peptides, Lane 2: Rat muscle, Lane 3: Mouse brain, Lane 4: A549 cells(heat-shock treatment), Lane 5: RAW264.7 cells(H2O2 treatment).
IHC
pan-AKT1/2/3 Antibody at 1/100 staining Human normal tissues adjacent to mammary cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.
ICC/IF
pan-AKT1/2/3 Antibody staining A549 cells by IF/ICC.The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab and mouse anti-beta tubulin Ab for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody. The nuclear counter stain is DAPI(blue).| Application Notes | WB:1:500-1:2000 IHC:1:50-1:200 IF/ICC:1:100-1:500 |
|---|
| Storage Instructions | Store at -20 °C. Stable for 12 months from date of receipt. |
|---|---|
| Storage Buffer | Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
