WB
Western Blot analysis of 22RV-1, H460, U87, mouse brain cells using Apelin Polyclonal Antibody. Secondary antibody was diluted at 1:20000IHC
Immunohistochemical analysis of paraffin-embedded rat-brain, antibody was diluted at 1:100ICC/IF
Immunofluorescence analysis of A549. 1,primary Antibody(red) was diluted at 1:200(4°C overnight). 2, Goat Anti Rabbit IgG (H&L) - Alexa Fluor 594 Secondary antibody was diluted at 1:1000(room temperature, 50min).3, DAPI(blue) 10min.| Product Name | Apelin Polyclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | The antiserum was produced against synthesized peptide derived from the C-terminal region of human APLN. AA range:28-77 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:500-1:2000 IHC:1:100-300 ICC:1:100-300 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | APLN |
|---|---|
| Gene Synonyms | APEL XNPEP2 |
| Gene Full Name | apelin |
| Gene Summary | his gene encodes a peptide that functions as an endogenous ligand for the G-protein coupled apelin receptor. The encoded preproprotein is proteolytically processed into biologically active C-terminal peptide fragments. These peptide fragments activate different tissue specific signaling pathways that regulate diverse biological functions including fluid homeostasis, cardiovascular function and insulin secretion. This protein also functions as a coreceptor for the human immunodeficiency virus 1. [provided by RefSeq, Feb 2016] |
| Molecular Weight(MW) | 16kDa |
| Cellular Localization | Secreted. Secreted>Extracellular space. |
| Entrez Gene | 8862 |
|---|
WB
Western Blot analysis of 22RV-1, H460, U87, mouse brain cells using Apelin Polyclonal Antibody. Secondary antibody was diluted at 1:20000
IHC
Immunohistochemical analysis of paraffin-embedded rat-brain, antibody was diluted at 1:100
ICC/IF
Immunofluorescence analysis of A549. 1,primary Antibody(red) was diluted at 1:200(4°C overnight). 2, Goat Anti Rabbit IgG (H&L) - Alexa Fluor 594 Secondary antibody was diluted at 1:1000(room temperature, 50min).3, DAPI(blue) 10min.| Application Notes | WB:1:500-1:2000 IHC:1:100-300 ICC:1:100-300 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 15°C to -25°C/1 year(Do not lower than -25°C) |
| Storage Buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
