WB
Western blot analysis of lysates from 1) Rat Brain Tissue, 2)HeLa , 3)A431, 4) PC12 cells, (Green) primary antibody was diluted at 1:1000, 4°over night, secondary antibody was diluted at 1:10000, 37° 1hour. (Red) Tubulin β Polyclonal Antibody antibody was diluted at 1:5000 as loading control, 4°over night,secondary antibody was diluted at 1:10000, 37° 1hour.IHC
Immunohistochemical analysis of paraffin-embedded Human-liver tissue. 1,GFAP Monoclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.ICC/IF
Immunofluorescence analysis of Hela cell. 1,AR Polyclonal Antibody(red) was diluted at 1:200(4° overnight). GFAP Monoclonal Antibody(green) was diluted at 1:200(4°overnight). 2, Goat Anti Rabbit Alexa Fluor 594 was diluted at 1:1000(room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 was diluted at 1:1000(room temperature, 50min).| Product Name | GFAP Mouse Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic Peptide of GFAP |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | ICC/IFIHCWB |
| WB:1:2000-1:5000 IHC:1:50-1:300 ICC:1:200 |
|
| Species Reactivity | HumanMouseRat |
| Purification | Affinity purified |
| Gene Symbol | GFAP |
|---|---|
| Gene Synonyms | ALXDRD |
| Gene Full Name | glial fibrillary acidic protein |
| Gene Summary | This gene encodes one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Oct 2008] |
| Molecular Weight(MW) | 45kDa |
| Cellular Localization | Cytoplasm . Associated with intermediate filaments. |
WB
Western blot analysis of lysates from 1) Rat Brain Tissue, 2)HeLa , 3)A431, 4) PC12 cells, (Green) primary antibody was diluted at 1:1000, 4°over night, secondary antibody was diluted at 1:10000, 37° 1hour. (Red) Tubulin β Polyclonal Antibody antibody was diluted at 1:5000 as loading control, 4°over night,secondary antibody was diluted at 1:10000, 37° 1hour.
IHC
Immunohistochemical analysis of paraffin-embedded Human-liver tissue. 1,GFAP Monoclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
ICC/IF
Immunofluorescence analysis of Hela cell. 1,AR Polyclonal Antibody(red) was diluted at 1:200(4° overnight). GFAP Monoclonal Antibody(green) was diluted at 1:200(4°overnight). 2, Goat Anti Rabbit Alexa Fluor 594 was diluted at 1:1000(room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 was diluted at 1:1000(room temperature, 50min).| Application Notes | WB:1:2000-1:5000 IHC:1:50-1:300 ICC:1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Storage Buffer | PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol. |
